Novel T-cell epitopes on Schistosoma japonicum SjP40 protein and their preventive effect on allergic asthma in mice
Novel T-cell epitopes on Schistosoma japonicum SjP40 protein and their preventive effect on allergic asthma in mice
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日本血吸虫SjP40蛋白T细胞新表位及其对小鼠过敏性哮喘的预防作用
DOI：
10.1002/eji.201545775
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发表时间：
2016-05-01
影响因子：
5.4
通讯作者：
Liu, Peimei
中科院分区：
文献类型：
--
作者：
Ren, Jiling;Hu, Lizhi;Liu, Peimei
过敏性哮喘是一种由Th2细胞免疫应答介导的慢性炎症性疾病。目前，基于免疫偏离的免疫疗法是哮喘的有吸引力的预防和治疗策略。许多研究表明，细胞内细菌感染如分枝杆菌及其组分可通过增强Th1应答抑制哮喘反应，而蠕虫感染及其蛋白可通过免疫调节抑制过敏性哮喘。然而，一些蠕虫蛋白如SmP40，曼氏血吸虫的主要卵抗原，被发现为Th1型抗原。利用一组重叠肽，我们确定了日本血吸虫SjP40蛋白的T细胞表位，它可以诱导Th1细胞因子，抑制Th2细胞因子的产生和过敏性哮喘小鼠模型的气道炎症。这些结果揭示了一种新的免疫保护机制，可能在蠕虫感染对过敏性哮喘反应的调节作用中发挥重要作用。
Allergic asthma is a chronic inflammatory disease mediated by Th2 cell immune responses. Currently, immunotherapies based on immune deviation are attractive, preventive, and therapeutic strategies for asthma. Many studies have shown that intracellular bacterial infections such as mycobacteria and their components can suppress asthmatic reactions by enhancing Th1 responses, while helminth infections and their proteins can inhibit allergic asthma via immune regulation. However, some helminth proteins such as SmP40, the major egg antigen of Schistosoma mansoni, are found as Th1 type antigens. Using a panel of overlapping peptides, we identified T-cell epitopes on SjP40 protein of Schistosoma japonicum, which can induce Th1 cytokine and inhibit the production of Th2 cytokines and airway inflammation in a mouse model of allergic asthma. These results reveal a novel form of immune protective mechanism, which may play an important role in the modulating effect of helminth infection on allergic asthmatic reactions.