EXPANSION OF ADIPOSE-TISSUE STORAGE CAPACITY AT DIFFERENT AGES IN RATS
EXPANSION OF ADIPOSE-TISSUE STORAGE CAPACITY AT DIFFERENT AGES IN RATS
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DOI：
10.1016/0026-0495(82)90112-3
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发表时间：
1982-01-01
影响因子：
9.8
通讯作者：
PETTERSSON, P
中科院分区：
文献类型：
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作者：
BJORNTORP, P;KARLSSON, M;PETTERSSON, P
采用多种技术相结合的方法，在Spraogue-Dawley和Osborne-Mendel大鼠中研究了随着生长和饮食、脂肪诱导的肥胖而扩大脂肪组织存储能力的问题。对成熟脂肪细胞进行计数和形态计量学测量。所有重细胞在胶原酶释放后计数，然后进行不同的培养程序。在防止细胞增殖的悬浮介质中培养等分部分，并假设允许通过在该系统中填充脂肪来估计体内确定的、未填充的前体细胞到脂肪细胞(前脂肪细胞)。简单的纯化步骤允许另一等量的重细胞在富含底物的培养基中以融合的单层形式增殖和形成脂肪细胞或前脂肪细胞，基本上是定量的。在融合之前，这些细胞不被确定为脂肪细胞系列中的细胞，因此被称为脂肪母细胞。通过测量标记胸苷掺入DNA的速率来确定它们的增殖率。所获得的结果与下面的综合图景相一致。幼年大鼠的脂肪组织是通过脂肪母细胞的增殖而生长的，在确定前脂肪细胞填满脂肪细胞后，脂肪母细胞填充到脂肪细胞，直到组织完全生长。除脂肪细胞外，其他细胞的数量随着年龄的增长而不断增加。在成年大鼠中，喂食脂肪会导致脂肪库的扩张，首先是通过增加脂肪细胞的大小。脂肪母细胞和脂肪细胞供应和支持细胞的形成是早期触发的。这种尚未招募到脂肪细胞的前体细胞池的准备状态是一个普遍现象。当增加的甘油三酯存储需求不再能通过可用脂肪细胞的扩张来满足时，可用脂肪细胞现在已经达到临界大小并已满，然后从脂肪母细胞前体池中招募细胞并确定成为前体脂肪细胞，这些前体脂肪细胞被快速填充甘油三酯以成熟脂肪细胞。这一决定可能是由局部信号触发的，并与细胞扩张的程度有关。与Osborne-Mendel大鼠相比，Spraogue-Dawley大鼠对脂肪喂养不那么敏感，而且只表现出这种发育的一部分。
Expansion of adipose tissue storage capacity with growth and with dietary, fat-induced obesity was studied in Sprague-Dawley and Osborne-Mendel rats by a combination of techniques. Mature adipocytes were counted and measured morphometrically. All heavy cells were counted after collagenase liberation and then subjected to different culture procedures. An aliquot part was cultured in a suspension medium which prevented cellular multiplication, and was assumed to allow an estimation of in vivo determined, non-filled precursor cells to adipocytes (preadipocytes) by their filling with lipid in this system. Simple purification steps allowed another aliquot of the heavy cells to multiply and form, essentially quantitatively, adipocytes or preadipocytes in a confluent monolayer in a substrate-rich culture-medium. Before confluence these cells are not determined to cells in the adipocytes series and were therefore called adipoblasts. Their rate of multiplication was determined by measurement of rate of incorporation of labeled thymidine into DNA. Results obtained are compatible with the following integrated picture. Adipose tissue of the young rat is growing by an increased multiplication of adipoblasts, which after determination to preadipocytes fill up to adipocytes until the tissue is full-grown. Cells other than adipocytes continue to increase in number with age. In the adult rat, fat-feeding results in expansion of adipose depots, first by increase in fat cell size. Formation of adipoblasts and cells for supply and support of adipocytes is triggered early. This readiness of the precursor pool of cells, not yet recruited to adipocytes, is a generalized phenomenon. When the increased demand for triglyceride storage can no longer be met by an expansion of available adipocytes, which now have reached a critical size and are full, then cells from the adipoblast precursor pool are recruited and are determined to become preadipocytes, which are rapidly filled with triglycerides to mature adipocytes. This determination is presumably triggered by a local signal and is associated with the degree of cellular expansion. Sprague-Dawley rats are less sensitive to fat-feeding than Osborne-Mendel rats and showed only part of this development.