Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus bacteria in oysters along the southwest coast of India
Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus bacteria in oysters along the southwest coast of India
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DOI：
10.1128/aem.71.7.3575-3580.2005
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发表时间：
2005-07-01
影响因子：
4.4
通讯作者：
Karunasagar, I
中科院分区：
文献类型：
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作者：
Deepanjali, A;Kumar, HS;Karunasagar, I
用非放射性标记的寡核苷酸探针进行菌落杂交，研究了印度西南海岸两个河口牡蛎中副溶血性弧菌的季节丰度。用与不耐热溶血素(Tlh)基因结合的探针测定副溶血性弧菌的总密度，用与耐热直接溶血素(Tdh)基因结合的探针测定致病性副溶血弧菌的密度。此外，通过聚合酶链式反应扩增毒素R、tdh和trh基因，研究了副溶血性弧菌的流行情况。直接用牡蛎匀浆进行聚合酶链式反应，然后在碱性蛋白调水中富集6h和18h。93.87%的样品检出副溶血性弧菌，其密度范围为
The seasonal abundance of Vibrio parahaemolyticus in oysters from two estuaries along the southwest coast of India was studied by colony hybridization using nonradioactive labeled oligonucleotide probes. The density of total V. parahaemolyticus bacteria was determined using a probe binding to the tlh (thermolabile hemolysin) gene, and the density of pathogenic V. parahaemolyticus bacteria was determined by using a probe binding to the tdh (thermostable direct hemolysin) gene. Furthermore, the prevalence of V. parahaemolyticus was studied by PCR amplification of the toxR, tdh, and trh genes. PCR was performed directly with oyster homogenates and also following enrichment in alkaline peptone water for 6 and 18 h. V. parahaemolyticus was detected in 93.87% of the samples, and the densities ranged from