Models of progression spanning preneoplasia and metastasis: the human MCF10AneoT.TGn series and a panel of mouse mammary tumor subpopulations.
Models of progression spanning preneoplasia and metastasis: the human MCF10AneoT.TGn series and a panel of mouse mammary tumor subpopulations.
复制标题
跨越肿瘤前期和转移的进展模型：人类 MCF10AneoT.TGn 系列和一组小鼠乳腺肿瘤亚群。
DOI：
10.1007/978-1-4613-1259-8_13
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发表时间：
1996
影响因子：
--
通讯作者：
Miller,FR
中科院分区：
文献类型：
--
作者：
Miller,FR
人类乳腺癌的进展通常被描述为雌激素受体表达的丧失，因为癌症从雌激素应答性/依赖性表型转变为雌激素非依赖性表型。这个定义的狭窄性源于这个阶段在预后和激素治疗方法方面的明显重要性。其他研究者倾向于将进展视为启动和促进事件、体外接触抑制和/或锚定依赖性的丧失、体外生长因子需求的变化、侵袭能力的获得、转移表型的获得或从增生性癌前病变转变为肿瘤性病变。当然，进展是所有这些事件的连续体，从正常上皮细胞的第一次改变，到癌前细胞，再到最终致命的转移细胞。我们已经开发了两个模型，跨越了整个进展谱，一个是人类癌前模型，另一个是一组密切相关的姐妹亚群，来自一个单一的小鼠乳腺肿瘤，通过不同的途径转移或未能转移在不同的转移顺序步骤。
Progression in human breast cancer is often depicted as the loss of estogen receptor expression as cancers shift from hormone-responsive/dependent to hormone-independent phenotypes. The narrowness of this definition stems from the obvious importance of this stage in progression in terms of prognosis and hormonal therapeutic approaches. Other investigators tend to view progression as initiation and promotion events, loss of contact inhibition and/or anchorage dependence in vitro, changes in growth factor requirements in vitro, acquirement of invasive capabilities, acquirement of metastatic phenotype, or transition from hyperplastic preneoplastic to neoplastic lesions. Of course, progression is a continuum of all these events from the first alteration of a normal epithelial cell, to the preneoplastic cell, to the ultimately lethal metastatic cell. We have developed two models that span the full spectrum of progression, one a human preneoplastic model and one a panel of closely related sister subpopulations derived from a single mouse mammary tumor that metastasize by different routes or fail to metastasize at different sequential steps of metastasis.