Dicer is required for maintaining adult pancreas.
Dicer is required for maintaining adult pancreas.
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维护成年胰腺需要迪切尔。
DOI：
10.1371/journal.pone.0004212
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Hatada I
中科院分区：
文献类型：
--
作者：
Morita S;Hara A;Kojima I;Horii T;Kimura M;Kitamura T;Ochiya T;Nakanishi K;Matoba R;Matsubara K;Hatada I
Dicer1是RNA干扰和微小RNA途径的关键组成部分，在发育组织的形态发生中具有许多重要作用。据报道，Dicer1基因缺失的小鼠在胚胎期7.5天死亡；因此无法研究其在成年组织中的功能。我们之前报道过，Dicer1亚效等位基因小鼠（其所有组织中的Dicer1表达降低至20%）出人意料地能够存活。在此我们对这些小鼠进行了分析，以确定Dicer1表达的下调是否对成年组织有任何影响。有趣的是，成年（8 - 10周龄）Dicer1亚效等位基因小鼠除胰腺外所有组织在组织学上均正常，其胰腺在胎儿和新生儿阶段发育正常；然而，在4周龄后在Dicer1亚效等位基因小鼠中检测到形态异常。这表明Dicer1对维持成年胰腺很重要。
Dicer1, an essential component of RNA interference and the microRNA pathway, has many important roles in the morphogenesis of developing tissues. Dicer1 null mice have been reported to die at E7.5; therefore it is impossible to study its function in adult tissues. We previously reported that Dicer1-hypomorphic mice, whose Dicer1 expression was reduced to 20% in all tissues, were unexpectedly viable. Here we analyzed these mice to ascertain whether the down-regulation of Dicer1 expression has any influence on adult tissues. Interestingly, all tissues of adult (8–10 week old) Dicer1-hypomorphic mice were histologically normal except for the pancreas, whose development was normal at the fetal and neonatal stages; however, morphologic abnormalities in Dicer1-hypomorphic mice were detected after 4 weeks of age. This suggested that Dicer1 is important for maintaining the adult pancreas.