INTERACTIONS AMONG PHOSPHATE UPTAKE, PHOTOSYNTHESIS, AND CHLOROPHYLL FLUORESCENCE IN NUTRIENT‐LIMITED CULTURES OF THE CHLOROPHYTE MICROALGA DUNALIELLA TERTIOLECTA 1
INTERACTIONS AMONG PHOSPHATE UPTAKE, PHOTOSYNTHESIS, AND CHLOROPHYLL FLUORESCENCE IN NUTRIENT‐LIMITED CULTURES OF THE CHLOROPHYTE MICROALGA DUNALIELLA TERTIOLECTA 1
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叶绿微藻 Dunaliella tertiolecta 营养限制培养物中磷酸盐吸收、光合作用和叶绿素荧光之间的相互作用 1
DOI：
10.1111/j.1529-8817.2008.00515.x
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发表时间：
2008
影响因子：
2.9
通讯作者：
J. Beardall
中科院分区：
文献类型：
--
作者：
S. Roberts;Kirsten Shelly;J. Beardall
研究了海洋叶绿微藻 Dunaliella tertiolecta Butcher 的磷酸盐限制和磷酸盐充足连续培养物对添加磷酸盐的反应。磷酸盐限制培养物在磷酸盐脉冲后显示出叶绿素荧光的显着猝灭。在磷酸盐充足的条件下生长的细胞不存在这种反应。荧光猝灭的程度（如果存在）和猝灭的初始变化率都取决于添加到细胞悬浮液中的磷酸盐的浓度和限制程度（连续培养中的生长速率）。磷酸盐的添加还导致光合产氧量的短暂减少和呼吸的刺激，随着添加的磷酸盐从外部介质中耗尽，这些现象得到缓解。讨论了使用营养诱导的荧光瞬变作为识别浮游植物种群营养状况的工具的适用性。
Phosphate‐limited and phosphate‐sufficient continuous cultures of the marine chlorophyte microalga Dunaliella tertiolecta Butcher were examined for their responses to the addition of phosphate. Phosphate‐limited cultures showed a marked quenching of chl fluorescence following a pulse of phosphate. This response was absent from cells growing under phosphate‐sufficient conditions. Both the extent of fluorescence quenching (where present) and the initial rate of change in quenching were dependent on the concentration of phosphate added to cell suspensions and on the degree of limitation (growth rate in continuous culture). The addition of phosphate also brought about a transient decrease in photosynthetic oxygen evolution and a stimulation in respiration, which were relaxed as the added phosphate was depleted from the external medium. The applicability of using nutrient‐induced fluorescence transients as a tool to identify the nutrient status of phytoplankton populations is discussed.