EVALUATION OF ACROSOMAL INTEGRITY OF BOAR SPERMATOZOA BY BRIGHT FIELD MICROSCOPY USING AN EOSIN-NIGROSIN STAIN
EVALUATION OF ACROSOMAL INTEGRITY OF BOAR SPERMATOZOA BY BRIGHT FIELD MICROSCOPY USING AN EOSIN-NIGROSIN STAIN
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DOI：
10.1016/0093-691x(88)90004-0
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发表时间：
1988-06-01
期刊：
影响因子：
2.8
通讯作者：
BAMBA, K
中科院分区：
文献类型：
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作者：
BAMBA, K
比较了三种伊红-苯胺黑染色溶液在估计新鲜和延长（冷冻和解冻）精液中精子顶体完整性的用途。染色液的渗透压和涂片/空气固定前精子暴露于染色液的时间是影响顶体形态的主要因素。长期冷冻/解冻精液的精子顶体完整性比新鲜精液更容易受到这些因素的影响。另一方面，染色溶液的 pH 值只是一个次要因素。在预先调节至生理 pH 值和渗透压的伊红-苯胺黑溶液中观察到具有完整顶体的非嗜酸性精子的最高百分比。该值也类似于使用微分干涉对比光学通过涂片确定的冻融精液顶体完整性的估计值。
Three eosoin-nigrosin staining solutions were compared regarding their use in estimating sperm acrosomal integrity in fresh and extended (frozen and thawed) semen. The osmolality of the staining solution and the duration of sperm exposure to the staining solution before smearing/air fixation were major factors affecting acrosomal morphology. Sperm acrosomal integrity of extended frozen/thawed semen was more vulnerable to these factors than was that of fresh semen. On the other hand, pH of the staining solutions was only a minor factor. The highest percentage of non-eosinophilic spermatozoa with an intact acrosome was observed in an eosin-nigrosin solution previously adjusted to physiological pH and osmolality. This value was also similar to an estimate of acrosomal integrity in frozen-thawed semen determined from smears using differential interference-contrast optics.