PHOSPHOLIPID BIOSYNTHESIS IN MEMBRANES OF IMMATURE AND MATURE RED BLOOD-CELLS
PHOSPHOLIPID BIOSYNTHESIS IN MEMBRANES OF IMMATURE AND MATURE RED BLOOD-CELLS
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DOI：
10.1021/bi00737a014
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发表时间：
1973-01-01
期刊：
影响因子：
2.9
通讯作者：
LENNARZ, WJ
中科院分区：
文献类型：
--
作者：
PERCY, AK;SCHMELL, E;LENNARZ, WJ
材料和方法放射性化合物和脂质。放射性化合物获自新英格兰核公司。脂质底物和标准品购自加拿大安大略省伦敦的 Serdary 研究实验室。 1, 2-甘油二酯和CDP-甘油二酯的脂肪酰基是棕榈酰基部分。网织红细胞和红细胞膜。血液取自新西兰白兔。如前所述制备红细胞膜（Burka 等人，1967）。每天皮下注射 1 ml 2.5% 苯肼的 0.1 m 磷酸钠缓冲液，pH 7.4（每天 10-15 mg/kg 体重），持续 4 天，诱导网织红细胞增多。最后一次注射后四十八小时，使用肝素注射器通过心脏穿刺获得血液。网织红细胞膜的制备方法与红细胞膜相同（Burka 等，1967）。用在 100 ml 水中含有 0.5 g 新亚甲基蓝和 1.6 g 草酸钾的溶液染色后，通过显微镜对网织红细胞进行定量。未经治疗的兔子的网织红细胞
Materials and MethodsRadioactive Compounds and Lipids. Radioactive compounds were obtained from New England Nuclear Corp. Lipid substrates and standards were purchased from Serdary Research Laboratories, London, Ontario, Can. The fatty acyl groups of the 1, 2-diglyceride and the CDP-diglyceride were palmitoyl moieties.Reticulocyte and Erythrocyte Membranes. Blood was obtained from New Zealand white rabbits. Erythrocyte mem-branes were prepared as previously described (Burka et a!., 1967). Reticulocytosis was induced by a daily, subcutaneous injection of 1 ml of 2.5% phenylhydrazine in 0.1 m sodium phosphate buffer, pH 7.4 (10-15 mg/kg body weightper day), for 4 days. Forty-eight hours after the final injection blood was obtained via cardiac puncture using a heparinized syringe. Reticulocyte membranes were prepared in the same manner as the erythrocyte membranes (Burka et al., 1967). Reticulocytes were quantitated by microscopy after staining with a solution containing 0.5 g of new Methylene Blue and 1.6 g of potassium oxalate in 100 ml of water. Reticulocytes in untreated rabbits