Analysis of receptor-ligand interactions by surface plasmon resonance.
Analysis of receptor-ligand interactions by surface plasmon resonance.
复制标题
通过表面等离子体共振分析受体-配体相互作用。
DOI：
10.1007/978-1-61779-139-0_6
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发表时间：
2011
影响因子：
--
通讯作者：
K. Maenaka
中科院分区：
文献类型：
--
作者：
K. Kuroki;K. Maenaka
许多免疫应答通常在细胞-细胞识别事件中由细胞表面受体调节。细胞表面上的这种免疫受体通常表现出低亲和力和快速动力学配体相互作用（例如，K（d）在μM范围内，k（off）= 10（-2）至20 s（-1））。实时表面等离子体共振（SPR）检测系统通常可用于确定这些结合参数。然而，应考虑几个技术要点，因为低亲和力结合和快速动力学的测定通常相当困难。在这里，我们介绍了SPR实验的一般程序，此外，显示典型的例子，免疫细胞表面受体的配体结合，包括实验上有用的提示。我们还展示了如何使用非线性范特霍夫和Arrhenius分析来确定热力学特性。这些免疫受体结合的亲和力、动力学和热力学参数对于理解免疫学事件以及开发药物和疫苗是重要的。
Many immunological responses are often regulated by cell surface receptors in cell-cell recognition events. Such immune receptors on the cell surface typically exhibit low-affinity and fast-kinetic ligand interactions (e.g., K (d) in the μM range, k (off) = 10(-2) to 20 s(-1)). Real-time surface plasmon resonance (SPR) detection systems are generally useful for determining these binding parameters. However, several technical points should be considered because the determination of low-affinity binding and fast kinetics is often rather difficult. Here, we introduce a general procedure for SPR experiments and, moreover, show typical examples for ligand binding of immune cell surface receptors, including experimentally useful tips. We also show how to determine the thermodynamic characteristics using the nonlinear van't Hoff and Arrhenius analyses. These affinity, kinetic, and thermodynamic parameters of immune-receptor binding are important for understanding immunological events as well as developing drugs and vaccines.
影响因子：
32.4
作者：
Ding, YH;Baker, BM;Wiley, DC
通讯作者：
Wiley, DC
DOI：
10.1073/pnas.89.8.3429
发表时间：
1992-04-15
影响因子：
11.1
作者：
GARBOCZI, DN;HUNG, DT;WILEY, DC
通讯作者：
WILEY, DC