Paper-based devices for rapid diagnostics and testing sewage for early warning of COVID-19 outbreak
Paper-based devices for rapid diagnostics and testing sewage for early warning of COVID-19 outbreak
复制标题
用于新冠肺炎疫情预警的纸质污水快速诊断和检测设备
DOI：
10.1016/j.cscee.2020.100064
复制
发表时间：
2020-11-29
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Hui Q;Pan Y;Yang Z
由SARS-CoV-2引起的冠状病毒病（COVID-19）于二零二零年演变为全球大流行病，疫情对全球个人、家庭、社区及社会造成巨大损失。一个切实有效的战略是实施基于快速检测的快速病例识别，以应对这一公共卫生危机。目前，用于快速诊断的可用技术包括RT-PCR、RT-LAMP、ELISA和NGS。尽管如此，由于其不同的局限性，它们并不适合在各种位置进行快速诊断。纸基设备是实现快速诊断的替代方法，其具有成本效益、高度选择性、灵敏、便携和易于使用。除了对单个病毒进行筛查外，基于废水的流行病学已成为对人群内疫情进行早期预警的有效方法，该方法检测病毒基因组序列，以反映病毒传播和分布的信息，因为SARS-CoV-2可以通过受感染人群的粪便和尿液进入废水。在本文中，我们描述了纸基设备作为一种低成本和快速的传感器，诊断和检测污水的早期预警爆发。更重要的是，该设备在现场实时检测方面具有巨大潜力，无需任何先进设施或训练有素的熟练人员，并对大流行病的爆发提供早期预警或及时干预。
Coronavirus disease (COVID-19), caused by SARS-CoV-2, evolved into a global pandemic in 2020, and the outbreak has taken an enormous toll on individuals, families, communities and societies around the world. One practical and effective strategy is to implement rapid case identification based on a rapid testing to respond to this public health crisis. Currently, the available technologies used for rapid diagnostics include RT-PCR, RT-LAMP, ELISA and NGS. Still, due to their different limitations, they are not well suited for rapid diagnosis in a variety of locations. Paper-based devices are alternative approaches to achieve rapid diagnosis, which are cost-effective, highly selective, sensitive, portable, and easy-to-use. In addition to individual virus screening, wastewater-based epidemiology has been emerged to be an effective way for early warning of outbreak within the population, which tests viral genome sequence to reflect information on the spread and distribution of the virus because SARS-CoV-2 can be shed into wastewater through the feces and urine from infected population. In this paper, we describe paper-based device as a low-cost and rapid sensor for both diagnosis and testing of sewage for early warning of outbreak. More importantly, the device has great potential for real-time detection in the field, without any advanced facilities or well-trained and skilled personnel, and provides early warning or timely intervention of an outbreak of pandemic.