A simple and rapid laboratory method for determination of haemostasis potential in plasma II.: Modifications for use in routine laboratories and research work
A simple and rapid laboratory method for determination of haemostasis potential in plasma II.: Modifications for use in routine laboratories and research work
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DOI：
10.1016/s0049-3848(01)00332-2
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发表时间：
2001-09-01
影响因子：
7.5
通讯作者：
Blombäck, M
中科院分区：
文献类型：
--
作者：
He, S;Antovic, A;Blombäck, M
为了提供一个合适的方法，用于常规实验室和研究工作，我们对早期开发的总止血潜力（OHP）测定法进行了一些修改。在血浆中加入降低剂量的凝血酶（伴或不伴组织型纤溶酶原激活剂），以启动纤维蛋白原凝血。两条纤维蛋白聚集曲线下的面积，即，因此产生了上述OHP和总凝固电位（OCP）。两个参数之间的差异反映了总体纤维蛋白溶解潜力（OFP），通过（（OCP-OHP）/OCP）× 100%计算。为了获得参考范围，对142名不同年龄的健康成年人和29名正常妊娠的健康妇女进行了调查。16例冠心病患者OCPs、OHPs升高，OFPs降低。在10例OCP中度增强的先兆子痫患者中，大多数OHP明显升高，而OFP降低。在因子VIII-、IV、VII-、V-、V或II-缺陷血浆样本中显示极低或不可检测水平的OHP和OCP。在13名接受乙酰水杨酸（阿萨）治疗的健康志愿者中，OHP在给药期间下降，停药后再次上升。上述结果表明，本研究中的修改使该方法更有效地检测止血变化，并与监测治疗相关。(C)2001爱思唯尔科技有限公司版权所有。
To offer a suitable method for use in routine laboratories and research work, some modifications were made in the assay of overall haemostatsis potential (OHP) we developed earlier. Thrombin in a decreased dose with or without tissue-type plasminogen activator was added to plasma for initiation of fibrinogen clotting. Areas under two fibrin-aggregation curves i.e., above-mentioned OHP and overall coagulation potential (OCP) were thus created. A difference between the two parameters reflects the overall fibrinolysis potential (OFP), calculated by ((OCP - OHP)/OCP) x 100%. To obtain reference ranges, investigations were performed in 142 healthy adults of different ages and in 29 healthy women with a normal pregnancy. In 16 patients suffering from coronary heart disease (CHD), OCPs and OHPs increased but OFPs decreased. In 10 pre-eclamptic women with moderate enhancement of OCP, the OHPs became noticeably higher in most while the OFPs lower. Extremely low or undetectable levels of OHP and OCP were shown in samples of Factors VIII-, IV, VII-, V-, V or II-deficient plasma. In 13 healthy volunteers treated with acetylsalicylic acid (ASA), OHPs expectedly declined during the administration and rose again after withdrawal. The above findings demonstrate that the modifications in the present study have rendered the method more effective for detecting haemostatic changes and relevant for monitoring treatments. (C) 2001 Elsevier Science Ltd. All rights reserved.