Localized colonic inflammation increases cytokine levels in distant small intestinal segments in the rat
Localized colonic inflammation increases cytokine levels in distant small intestinal segments in the rat
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DOI：
10.1016/j.lfs.2006.06.047
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发表时间：
2006-10-19
期刊：
影响因子：
6.1
通讯作者：
Saade, Nayef E.
中科院分区：
文献类型：
--
作者：
Barada, Kassem A.;Mourad, Fadi H.;Saade, Nayef E.
结肠的局部炎症与小肠的营养吸收不良和动力改变有关。这些远程效应表明介质的释放可以作用于（或改变）远离原发炎症区域的肠段的功能。本研究描述了两种实验性结肠炎大鼠模型中结肠和小肠各段促炎细胞因子表达的变化。通过结肠内施用100 μ L的6%碘乙酰胺或250 μ L的2，4，6-三硝基苯磺酸诱导结肠炎。在不同的时间间隔从十二指肠、空肠、回肠和结肠取样的组织匀浆中通过ELISA测量白细胞介素1 β、白细胞介素6和肿瘤坏死因子α的水平。在从十二指肠、空肠和回肠分离的条的匀浆中，肿瘤坏死因子α和白细胞介素-6在碘乙酰胺或TNBS施用后3-6小时显著增加，并且保持升高直到结肠炎症消退。白细胞介素1 β显示出相当但延迟的增加。类似的，但更明显的增加，三种细胞因子被注意到在邻近溃疡的结肠区域。组织学检查显示结肠中存在重要的炎症变化;然而，小肠切片检查未显示对照组和结肠炎大鼠之间存在显著差异。总之，在结肠炎期间，促炎细胞因子的表达在小肠的远端节段中增加。这些发现可能为相关的小肠功能障碍提供部分解释或分子底物。(c)2006年爱思唯尔公司All rights reserved.
Local inflammation in the colon has been associated with nutrient malabsorption and altered motility in the small bowel. These remote effects suggest the release of mediators which can act (or alter) the function of intestinal segments located far from the primary area of inflammation. This study describes the changes in the expression of pro-inflammatory cytokines in the colon and in various segments of the small intestine in two rat models of experimental colitis. Colitis was induced by the intracolonic administration of 100 mu L of 6% iodoacetamide or 250 mu L of 2, 4, 6-trinitrobenzene sulfonic acid. Levels of interleukin one beta, interleukin 6, and tumor necrosis factor alpha were measured by ELISA in tissue homogenate sampled from duodenum, jejunum, ileum and colon at different time intervals. In homogenates of strips isolated from duodenum, jejunum and ileum, tumor necrosis alpha and interleukin-6, increased significantly 3-6 h after iodoacetamide or TNBS administration and remained elevated until the colonic inflammation subsided. Interleukin one beta showed comparable but delayed increase. Similar, but more pronounced increase of the three cytokines was noticed in areas of the colon adjacent to the ulcer. Histologic examinations revealed important inflammatory changes in the colon; however, examination of sections from the small intestines did not reveal significant differences between controls and rats with colitis. In conclusion, expression of pro-inflammatory cytokines is increased in remote segments of the small intestines during colitis. The findings may provide a partial explanation or a molecular substrate for the associated small bowel dysfunction. (c) 2006 Elsevier Inc. All rights reserved.