Propionate modulates spontaneous contractions via enteric nerves and prostaglandin release in the rat distal colon
Propionate modulates spontaneous contractions via enteric nerves and prostaglandin release in the rat distal colon
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DOI：
10.2170/jjphysiol.rp000205
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发表时间：
2005-12-01
期刊：
影响因子：
--
通讯作者：
Kuwahara, A
中科院分区：
文献类型：
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作者：
Mitsui, R;Ono, S;Kuwahara, A
短链脂肪酸，例如丙酸和乙酸，是通过结肠腔中碳水化合物的细菌发酵产生的。我们研究了丙酸盐对附有粘膜的大鼠远端结肠环形肌条中自发性巨大收缩（GC）的频率和平均幅度的影响。添加丙酸盐可增加 GC 频率约 20 分钟 (>= 1 mm)，但平均振幅降低 (>= 0.1 mm)。丙酸诱导的 GC 频率增加被毒蕈碱乙酰胆碱受体拮抗剂阿托品阻断。相比之下，烟碱受体拮抗剂六甲铵则增强了反应。环加氧酶抑制剂吡罗昔康可防止丙酸盐诱导的 GC 平均振幅降低。用乙酸盐预处理组织可防止丙酸盐诱导的 GC 频率和振幅调节。这些结果表明，丙酸盐通过激活胆碱能运动神经元来增加 GC 的频率，并通过前列腺素的释放来降低平均振幅。丙酸盐和乙酸盐可能参与大鼠远端结肠自发环肌活动的调节。
Short-chain fatty acids, such as propionate and acetate, are produced by a bacterial fermentation of carbohydrates in the colonic lumen. We examined the effects of propionate on the frequency and mean amplitude of spontaneous giant contractions (GCs) in circular muscle strips of the rat distal colon with the mucosa attached. An addition of propionate increased the frequency of GCs for about 20 min (>= 1 mm), but the mean amplitude was decreased ( >= 0.1 mm). The propionate-induced increase in the frequency of GCs was blocked by the muscarinic acetylcholine receptor antagonist, atropine. In contrast, the nicotinic receptor antagonist, hexamethonium, augmented the response. The propionate-induced decrease in the mean amplitude of GCs was prevented by the cyclooxygenase inhibitor, piroxicam. A pretreatment of the tissues with acetate prevented the propionate-induced modulations of the frequency and amplitude of GCs. These results suggest that propionate increases the frequency of GCs by an activation of cholinergic motor neurons and decreases the mean amplitude by a prostaglandin release. Propionate as well as acetate may be involved in the regulation of spontaneous circular muscle activity in the rat distal colon.