Junctional Adhesion Molecule: An Expression in Human Endometrial Carcinoma
Junctional Adhesion Molecule: An Expression in Human Endometrial Carcinoma
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DOI：
10.1111/igc.0b013e31819bc6e9
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发表时间：
2009-02-01
影响因子：
4.8
通讯作者：
Honjo, Hideo
中科院分区：
文献类型：
--
作者：
Koshiba, Hisato;Hosokawa, Kenichi;Honjo, Hideo
连接粘附分子A（Junctional adhesion molecule A，JAM-A）参与细胞间的接触和紧密连接的形成。细胞粘附分子的缺失可能与子宫内膜癌的高组织学分级和侵袭性有关。我们试图确定JAM-A在人子宫内膜癌中的表达及其与病理特征、分期和生存率的相关性。应用免疫组化方法检测子宫内膜癌组织中连接粘附分子A的表达。此外，我们在三维基底膜制备中培养了人类高分化和低分化子宫内膜腺癌细胞系、石川细胞和KLE，并通过实时逆转录-聚合酶链反应和免疫组化评估了这些细胞中的JAM-A表达。连接黏附分子A免疫染色强度与组织学分级（tau =-0.420，P < 0.0001）、肌层浸润（tau =-0.306，P < 0.01）和分期（tau =-0.383，P < 0.0001）呈负相关。JAM-A免疫染色强度低与阳性血管间隙受累有关（P < 0.01）。低免疫染色强度与低总生存率和低无进展生存率显著相关（P < 0.0001）。此外，在我们的三维上皮细胞培养中，低分化腺癌中的JAM-A表达显著低于高分化腺癌（P < 0.001）。高级别或进展期子宫内膜癌中连接粘附分子A表达减少，可能是一个预后因素。
Junctional adhesion molecule A (JAM-A) is involved in cell-cell contact and tight junction formation. Loss of cell adhesion molecules may be associated with high histologic grade and invasiveness of endometrial carcinoma. We attempted to determine JAM-A expression in human endometrial carcinoma and its correlations with pathologic features, stage, and survival. Junctional adhesion Molecule A expression in human endometrial carcinoma was evaluated by immunohistochemistry. In addition, we cultured human well and poorly differentiated endometrial adenocarcinoma cell lines, Ishikawa cells, and KLE in 3-dimensional basement membrane preparation, and JAM-A expression in these cells was assessed by real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Junctional adhesion molecule A immunostaining intensity was negatively correlated with histologic grade (tau = -0.420, P < 0.0001), myometrial invasion (tau = -0.306, P < 0.01) and stage (tau = -0.383, P < 0.0001). Low JAM-A immunostaining intensity was associated With Positive Vascular space involvement (P < 0.01). Moreover, low immunostaining intensity was significantly (P < 0.0001) related to low overall survival rate and progression-free survival rate. Additionally, in our 3-dimensional epithelial cell culture, JAM-A expression in poorly differentiated adenocarcinoma was significantly lower than that in well-differentiated adenocarcinoma (P < 0.001). Junctional adhesion molecule A expression seems to be reduced in high-grade or advanced endometrial carcinoma and may be a prognostic factor.