A method to produce microseed stock for use in the crystallization of biological macromolecules
A method to produce microseed stock for use in the crystallization of biological macromolecules
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DOI：
10.1107/s0907444999002085
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发表时间：
1999-05-01
期刊：
影响因子：
--
通讯作者：
DeTitta, GT
中科院分区：
文献类型：
--
作者：
Luft, JR;DeTitta, GT
提出了一种制备大分子结晶用混合种子的方法。聚四氟乙烯珠和微离心管作为研钵和杵粉碎种子晶体的大分子。头部运动的能量由涡流混合器或超声波浴提供。将粉碎的晶体连续稀释，以制备所需浓度的种子原料混合物，用于结晶。用悬垂滴气扩散法和毛细管微批法制备的晶体均表现出预期的稀释行为。并与传统方法进行了比较，论证了该技术相对于标准方案的优越性。
A method is presented for producing a seed-stock mixture for macromolecular crystallization. A PTFE bead and microcentrifuge tube act as mortar and pestle for pulverizing seed crystals of macromolecules. Energy for the bead's motion is supplied by a vortex mixer or an ultrasonic bath. The crushed crystal is serially diluted to prepare a seed-stock mixture of the desired concentration for crystallization. Crystals produced using both hanging-drop vapor diffusion and a capillary microbatch method show expected dilution behavior. This technique of producing seed stock is compared with traditional means and advantages over the standard protocol are demonstrated.