ACTIN ANTIBODY - SPECIFIC VISUALIZATION OF ACTIN-FILAMENTS IN NON-MUSCLE CELLS
ACTIN ANTIBODY - SPECIFIC VISUALIZATION OF ACTIN-FILAMENTS IN NON-MUSCLE CELLS
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DOI：
10.1073/pnas.71.6.2268
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
WEBER, K
中科院分区：
文献类型：
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作者：
LAZARIDES, E;WEBER, K
用十二烷基硫酸钠凝胶电泳法从小鼠成纤维细胞中纯化肌动蛋白作为抗原，获得兔抗体。通过免疫扩散和补体固定对部分纯化的小鼠成纤维细胞肌动蛋白和高度纯化的鸡肌动蛋白具有特异性。该抗体用于间接免疫荧光，通过荧光显微镜显示各种细胞类型中含有肌动蛋白的纤维的分布和模式。肌动蛋白丝可以跨越细胞长度，或者集中在每个细胞特有的“焦点”上。
Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified chicken muscle actin. The antibody was used in indirect immunofluorescence to demonstrate by fluorescence light microscopy the distribution and pattern of actin-containing filaments in a variety of cell types. Actin filaments were shown to span the cell length or to concentrate in “focal points” in patterns characteristic for each individual cell.