Modes of correlated angular motion in live cells across three distinct time scales.
Modes of correlated angular motion in live cells across three distinct time scales.
复制标题
活细胞在三个不同时间尺度上的相关角运动模式。
DOI：
10.1088/1478-3975/10/3/036002
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发表时间：
2013
期刊：
影响因子：
2
通讯作者：
Harrison AW
中科院分区：
文献类型：
--
作者：
Harrison AW
使用高速数字显微镜的粒子跟踪实验产生活细胞内脂滴的位置和轨迹。角相关分析表明，脂滴在短时间尺度（τ< 1 ms）下具有不相关运动，随后在1 μ τ ≤ 10 ms的滞后时间范围内具有反持续运动。在较长时间尺度（τ> 10 ms）下的角相关性变得持续，表明定向运动。在所有时间尺度上的运动都与脂滴被拴在微管网络上并被沿着微管网络驱动有关。角相关性从反持续运动变为持续运动的点对应于亚扩散运动和超扩散运动之间的交叉，如通过均方位移分析所观察到的。正确分析的角度相关性的检测器噪声被发现是至关重要的建模所观察到的现象。
Particle tracking experiments with high speed digital microscopy yield the positions and trajectories of lipid droplets inside living cells. Angular correlation analysis shows that the lipid droplets have uncorrelated motion at short time scales (τ< 1 ms) followed by anti-persistent motion for lag times in the range of 1⩽ τ⩽ 10 ms. The angular correlation at longer time scales, τ> 10 ms, becomes persistent, indicating directed movement. The motion at all time scales is associated with the lipid droplets being tethered to and driven along the microtubule network. The point at which the angular correlation changes from anti-persistent to persistent motion corresponds to the cross over between sub-diffusive and super diffusive motion, as observed by mean square displacement analysis. Correct analysis of the angular correlations of the detector noise is found to be crucial in modelling the observed phenomena.