RNA length defines RNA export pathway
RNA length defines RNA export pathway
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DOI：
10.1101/gad.1216204
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发表时间：
2004-09-01
影响因子：
10.5
通讯作者：
Ohno, M
中科院分区：
文献类型：
--
作者：
Masuyama, K;Taniguchi, I;Ohno, M
不同的 RNA 种类通过不同的机制从细胞核中输出。在不同的 RNA 中，mRNA 和主要剪接体 U snRNA 具有一些结构相似性，但它们是通过不同的因素输出的。我们之前表明，如果将各种类似的 300 个核苷酸的片段插入到中心位置，U1 snRNA 在核输出中的行为就像 mRNA 一样。在这里，我们证明这个输出开关取决于插入的长度，但与其位置无关，这明确表明这个开关确实是RNA长度的结果。我们还表明，如果 mRNA 通过删除逐渐缩短，则无内含子 mRNA 可以逐渐转换为使用 U snRNA 输出途径。此外，免疫沉淀实验表明，输出 RNP 的蛋白质组成受 RNA 长度的影响。这些发现表明RNA长度是RNA输出途径选择的关键决定因素之一。基于这些结果和之前的观察，提出了 RNA 如何致力于特定输出途径的统一模型。
Different RNA species are exported from the nucleus by distinct mechanisms. Among the different RNAs, mRNAs and major spliceosomal U snRNAs share several structural similarities, yet they are exported by distinct factors. We previously showed that U1 snRNAs behaved like an mRNA in nuclear export if various similar to300-nucleotide fragments were inserted in a central position. Here we show that this export switch is dependent on the length of the insertion but independent of its position, indicating unequivocally that this switch is indeed the result of RNA length. We also show that intronless mRNAs can be progressively converted to use the U snRNA export pathway if the mRNAs are progressively shortened by deletion. In addition, immunoprecipitation experiments show that the protein composition of export RNPs is influenced by RNA length. These findings indicate that RNA length is one of the key determinants of the choice of RNA export pathway. Based on these results and previous observations, a unified model of how an RNA is committed to a specific export pathway is proposed.