Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture
Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture
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DOI：
10.3390/molecules25030709
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发表时间：
2020-02
期刊：
影响因子：
4.6
通讯作者：
T. Steliga;K. Wójtowicz;P. Kapusta;J. Brzeszcz
中科院分区：
文献类型：
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作者：
T. Steliga;K. Wójtowicz;P. Kapusta;J. Brzeszcz
生物降解是消除环境中有毒多氯联苯（PCBs）和总石油烃（TPH）的最有效和最有利可图的方法之一。在这项研究中，上述污染物的细菌菌株Mycolicibacterium frederiksbergense IN 53，红串红球菌IN 129，红球菌属物种IN 306和混合培养物M1开发的基础上，以1：1：1的比例进行了好氧降解进行了分析。根据进行的呼吸测定试验和色谱分析，评估了单个菌株和混合培养物的有效性。红球菌IN306对18种多氯联苯同系物的生物降解率最高（54.4%）。随着分子中氯原子数的增加，生物降解指数逐渐降低。而Frederiksbergense分枝杆菌IN 53是最好的TPH降解菌（37.2%）。在无菌土壤中，污染的多氯联苯和TPH，最高的生物降解效率获得接种与混合培养M1，这使得减少两个PCBs（51.8%）和TPH（34.6%）的含量。采用棱镜法，在受老化石油烃（TPH）和废Transformer油（PCBs）污染的非无菌土壤中，对确定的混合菌M1的PCBs和TPH生物降解能力进行了异位验证。接种混合菌M1后，6个月内PCBs和TPH的去除率分别为84.5%和70.8%，土壤毒性也有所降低。
Biodegradation is one of the most effective and profitable methods for the elimination of toxic polychlorinated biphenyls (PCBs) and total petroleum hydrocarbons (TPH) from the environment. In this study, aerobic degradation of the mentioned pollutants by bacterial strains Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN129, and Rhodococcus sp. IN306 and mixed culture M1 developed based on those strains at 1:1:1 ratio was analyzed. The effectiveness of individual strains and of the mixed culture was assessed based on carried out respirometric tests and chromatographic analyses. The Rhodococcus sp. IN306 turned out most effective in terms of 18 PCB congeners biodegradation (54.4%). The biodegradation index was decreasing with an increasing number of chlorine atoms in a molecule. Instead, the Mycolicobacterium frederiksbergense IN53 was the best TPH degrader (37.2%). In a sterile soil, contaminated with PCBs and TPH, the highest biodegradation effectiveness was obtained using inoculation with mixed culture M1, which allowed to reduce both the PCBs (51.8%) and TPH (34.6%) content. The PCBs and TPH biodegradation capacity of the defined mixed culture M1 was verified ex-situ with prism method in a non-sterile soil polluted with aged petroleum hydrocarbons (TPH) and spent transformer oil (PCBs). After inoculation with mixed culture M1, the PCBs were reduced during 6 months by 84.5% and TPH by 70.8% as well as soil toxicity was decreased.