Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
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DOI：
10.2225/vol8-issue3-fulltext-6
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发表时间：
2005-12-01
影响因子：
2.7
通讯作者：
Kurina, Marcela
中科院分区：
文献类型：
--
作者：
Hernandez, Xenia E;Orden, Alejandro A;Kurina, Marcela
从植物幼苗叶片中建立了美丽草愈伤组织和细胞悬浮培养体系。当在含有3%（w/v）蔗糖的固体Murashige和Skoog培养基（MS培养基）中测定几种植物调节剂时，吲哚-3-丁酸（IBA）和N-6-苄基氨基嘌呤（BA）的组合产生了最合适的条件以产生具有可检测水平的研磨酸的快速生长的易碎愈伤组织。此外，在相同的基础培养基中添加20.0 μ M IBA/4.4 μ M BA对深层培养的细胞生长最有利（mu（max）= 0.26天（-1）），而添加20.0 μ M IBA/18.0 μ M BA导致相对较高的代谢产物产量（4.55 mg/gDW），当接种量为5%（v/v）时。此外，三种不同的应力因素和它们的组合被用来引发细胞悬浮液。这些实验表明，CuSO 4和二甲亚砜（DMSO）的组合在引发试验中将研磨酸产量增加至2.63 mg/gDW，而在对照中为0.756 mg/gDW，以细胞生长为代价。与此相反，茉莉酸（JA）单独和与DMSO组合的添加既不影响细胞生长，也不影响grindelic酸积累。
Grindelia pulchella callus and cell suspension cultures were established from seedling leaves. When several phytoregulator supplementations were assayed in solid Murashige and Skoog medium containing 3% (w/v) of sucrose (MS medium), combinations of indole-3-butyric acid (IBA) and N-6-benzylaminopurine (BA) resulted the most appropriate conditions to generate fast growing friable calli with detectable levels of grindelic acid. Moreover, the same basal media supplemented with 20.0 mu M IBA/4.4 mu M BA was found to be optimal for cell growth in submerged cultures (mu(max) = 0.26 days(-1)) while the addition of 20.0 mu M IBA/18.0 mu M BA resulted in a relative higher metabolite production (4.55 mg/gDW) when the inocula was 5% (v/v). Furthermore, three different stress factors and combinations of them were used to elicit cell suspensions. These experiments demonstrated that the combination of CuSO4 and dimethylsulfoxide (DMSO) increase the grindelic acid production to 2.63 mg/gDW in the elicited essay versus 0.756 mg/gDW in the control, at expense of cell growth. In contrast, the addition of jasmonic acid (JA) alone and combined with DMSO neither affected cell growth nor grindelic acid accumulation.