In vitro effect of nerve growth factor on the main traits of rabbit sperm
In vitro effect of nerve growth factor on the main traits of rabbit sperm
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DOI：
10.1186/s12958-019-0533-4
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发表时间：
2019-11-12
影响因子：
4.4
通讯作者：
Rende, Mario
中科院分区：
文献类型：
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作者：
Castellini, Cesare;Mattioli, Simona;Rende, Mario
背景神经生长因子（nerve growth factor，NGF）是神经营养因子家族的一员，不仅在神经系统中发挥重要作用，而且在生殖系统等非神经系统中也发挥重要作用。本文旨在研究神经生长因子（NGF）对家兔精子功能的影响。方法分五次采集10只成年巴克斯，并对合并精液样本进行分析。定量精浆中的NGF，并确定精子中NGF受体（TrKA和p75 NTR）的分布。此外，还观察了NGF对运动率和跟踪速度的剂量效应。随后，评估了NGF受体的中和作用，以验证每种受体的特异性作用。未处理的精子用作对照。结果：（1）在精浆中检测到NGF，在精子表面检测到TrKA和p75 NTR。特别是，TrKA定位于头部，p75 NTR定位于兔精子的中段和尾部。ii）一旦确定了NGF的最佳剂量（100 ng/mL），其添加影响兔精子的动力学和其他生理特性（获能、凋亡和坏死）。(iii)TrKA和p75 NTR受体的中和作用对精子性状的影响不同。特别是，精子的速度，凋亡和获能似乎主要通过p75 NTR受体调制，而运动，活细胞，坏死和顶体反应调制通过TrKA。结论首次在家兔精子中发现了p75 NTR的存在。神经生长因子影响兔精子的动力学和其他生理特性。大多数这些变化是由相关受体（TrKA或p75 NTR）调节的。考虑到人类的一些精液疾病与较低的NGF浓度相关，并且没有对NGF受体可能参与的研究，这些发现也为人类生育提供了新的见解。
Background The nerve growth factor (NGF), a member of the neurotrophins family, plays an important role not only in the nervous but also in other non-nervous systems such as the reproductive system. The aim of the paper is to study the in vitro effect of NGF on rabbit sperm functions. Methods Ten adult rabbit bucks were collected five times, and pooled semen samples have been analysed. NGF was quantified in seminal plasma, and the distribution of NGF receptors (TrKA and p75NTR) in sperm was established. Moreover, the dose-effect of NGF on motility rate and track speed was evaluated. Successively, the effect of the neutralisation of NGF receptors was assessed to verify the specific role of each receptor. Untreated sperm were used as control. Results Our study identified several interesting results: i) We detected NGF in seminal plasma and TrKA and p75NTR in sperm surface. In particular, TrKA is localised in the head and p75NTR in the midpiece and tail of rabbit sperm. ii) Once the optimal dose of NGF (100 ng/mL) was established, its addition affected both kinetics and other physiological traits (capacitation, apoptosis and necrosis) of rabbit sperm. (iii) The neutralisation of TrKA and p75NTR receptors affected sperm traits differently. In particular, sperm speed, apoptosis and capacitation seemed mainly modulated via p75NTR receptor, whereas motile, live cells, necrosis and acrosome reaction were modulated via TrKA. Conclusion For the first time, we showed the presence of p75NTR in rabbit sperm. NGF affects kinetic and other physiological traits of rabbit sperm. Most of these changes are modulated by the receptors involved (TrKA or p75NTR). Considering that some seminal disorders in human have been correlated with a lower NGF concentration and no studies have been done on the possible involvement of NGF receptors, these findings also provide new insights on human fertility.