INTRACELLULAR CALCIUM-IONS DECREASE THE AFFINITY OF THE GABA RECEPTOR
INTRACELLULAR CALCIUM-IONS DECREASE THE AFFINITY OF THE GABA RECEPTOR
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DOI：
10.1038/324156a0
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发表时间：
1986-11-13
期刊：
影响因子：
64.8
通讯作者：
AKAIKE, N
中科院分区：
文献类型：
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作者：
INOUE, M;OOMURA, Y;AKAIKE, N
细胞内游离钙离子([Ca~(2+)]i)在细胞外信号转导中起着至关重要的作用。钙离子通量还参与交感神经节中烟碱能神经传递的突触后易化，以及乙酰胆碱(ACh)受体的激活。[Ca2+]i也是许多生物活性物质8的第二信使。我们记录了牛蛙(Rana Catesbiana)感觉神经元的神经元活动，用吸管吸管法9和“浓度钳”技术10对细胞施加γ-氨基丁酸(GABA)。我们首次报道了[Ca~(2+)]通过降低GABA受体的表观亲和力而抑制GABA激活的Cl-−电导的证据。
Intracellular free Ca2+([Ca2+]i) plays a crucial role in the transduction of extracellular signals1. It has been implicated in the modulation of light sensitivity inLimulusphotoreceptors2and in the efficacy of synaptic transmission3–5; calcium ion fluxes are also involved in the postsynaptic facilitation of nicotinic transmission seen in sympathetic ganglia, and in activation of the acetylcholine (ACh) receptor6,7. [Ca2+]iis also a second messenger for many biologically active substances8. We recorded neuronal activities of sensory neurones from the bullfrog (Rana catesbiana), using the suction pipette method9and a ‘concentration clamp’ technique10to apply γ-aminobutyric acid (GABA) to the cell. We report the first evidence that [Ca2+]isuppresses the GABA-acti-vated Cl−conductance, by decreasing the apparent affinity of the GABA receptor.