Determination of low-molecular-mass antioxidant concentrations in human respiratory tract lining fluids
Determination of low-molecular-mass antioxidant concentrations in human respiratory tract lining fluids
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DOI：
10.1152/ajplung.1999.276.2.l289
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发表时间：
1999-02-01
影响因子：
4.9
通讯作者：
Louie, S
中科院分区：
文献类型：
--
作者：
van der Vliet, A;O'Neill, CA;Louie, S
Antioxidants present within lung epithelial lining fluids (ELFs) constitute an initial line of defense against inhaled environmental oxidants such as ozone, nitrogen oxides, and tobacco smoke, but the antioxidant composition of human ELFs is still incompletely characterized. We analyzed ELF concentrations of the low-molecular-mass antioxidants ascorbate, urate, glutathione (GSH), and alpha-tocopherol by obtaining bronchoalveolar lavage (BAL) and nasal lavage fluids from healthy nonsmoking volunteers and compared two different BAL procedures. ELF dilution by the lavage procedures was estimated by measurement of urea in recovered BAL fluids in comparison with those in blood plasma from the same subjects. The results indicated that a recently developed, single-cycle BAL procedure minimizes influx of non-ELF urea into the instilled fluid and thus allows for a more accurate determination of ELF antioxidant concentrations. Using this procedure, we determined that bronchoalveolar ELF contains 40 +/- 18 (SD) mu M ascorbate, 207 +/- 167 mu M urate, 109 +/- 64 mu M GSH, and 0.7 +/- 0.3 mu M alpha-tocopherol (n = 12 subjects). Similar analysis of nasal lavage fluid yielded nasal ELF levels of 28 +/- 19 mu M ascorbate and 225 +/- 105 mu M urate (n = 12 subjects), whereas GSH was undetectable (
