Mechanisms of poly-N-acetyl glucosamine polymer-mediated hemostasis: Platelet interactions
Mechanisms of poly-N-acetyl glucosamine polymer-mediated hemostasis: Platelet interactions
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DOI：
10.1097/01.ta.0000136743.12440.89
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Fischer, TH
中科院分区：
文献类型：
--
作者：
Thatte, HS;Zagarins, S;Fischer, TH
背景资料：进行调查，以确定是否聚-N-乙酰氨基葡萄糖（p-GlcNAc）诱导止血的激活platelet.Methods：血小板分离自人血，固定在聚-N-乙酰氨基葡萄糖纤维的存在下，和可视化与扫描电子显微镜。用荧光多光子显微镜检测血小板活化表面标志物。在p-GlcNAc纤维和整合素受体阻滞剂存在下测定血小板聚集。扫描电子显微镜观察表明，血小板与聚-N-乙酰葡糖胺纤维接触导致血小板活化。荧光显微镜显示，血小板与海洋聚合物的接触增加了细胞内游离钙的水平，并导致血小板磷脂酰丝氨酸，P选择素和α（IIb）β（3）整合素的表面暴露。血小板α（IIb）β（3）整合素的抗体抑制剂抑制p-GlcNAc刺激纤维蛋白聚合。结论：聚-N-乙酰氨基葡萄糖纤维材料通过激活血小板促进止血。
Background: Investigations were performed to determine whether poly-N-acetyl glucosamine (p-GlcNAc) induces hemostasis by the activation of platelets.Methods: Platelets were isolated from human blood, fixed in the presence poly-N-acetyl glucosamine fibers, and visualized with scanning electron microscopy. Platelet activation surface markers were measured by fluorescence multiphoton microscopy. Platelet aggregation in the presence of p-GlcNAc fibers and integrin receptor blockers was measured.Results. Scanning electron microscopy indicated that contact of platelets with poly-N-acetyl glucosamine fibers resulted in platelet activation. Fluorescent microscopy showed that contact of platelets with the marine polymer increased intracellular levels of free calcium and resulted in surface exposure of platelet phosphatidylserine, P selectin, and the alpha(IIb)beta(3) integrin. Antibody inhibitors of the platelet alpha(IIb)beta(3) integrin inhibited p-GlcNAc to stimulate fibrin polymerization.Conclusion: Poly-N-acetyl glucosamine fiber material promotes hemostasis by the activation of platelets.