DNA replication initiates non-randomly at multiple sites near the c-myc gene in HeLa cells.
DNA replication initiates non-randomly at multiple sites near the c-myc gene in HeLa cells.
复制标题
DNA 复制在 HeLa 细胞中 c-myc 基因附近的多个位点非随机启动。
DOI：
10.1093/nar/24.10.1887
复制
发表时间：
1996
影响因子：
14.9
通讯作者：
Leffak,M
中科院分区：
文献类型：
--
作者：
Waltz,SE;Trivedi,AA;Leffak,M
在HeLa细胞中，c-myc基因的复制起源是基于复制叉极性和DNA新生链的位置，在3.5 kb 5 '到p1启动子的低分辨率下确定的。为了更高分辨率地定义c-myc起源的起始事件，我们利用链特异性探针杂交分析了12kb c-myc结构域新生dna的模板偏差。新生链模板偏好的不对称性的强烈开关证实了复制在距离c-myc p1启动子2.4 kb 5 '内的多个位点上非随机启动，以及在距离c-myc p1启动子12 kb或更长区域内的其他位点上非随机启动。最强的模板偏差发生在p1启动子的2.4 kb区域5 '，前面显示包含允许c-myc质粒自主半保守复制的序列。在c-myc起源区发现了一个不对称的嘧啶七核苷酸一致序列，该序列出现了12次，其极性与新生链合成的极性完全相关。
The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5′ to the P1promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DNAs in a 12 kb c-myc domain has been analyzed by hybridization to strand specific probes. Strong switches in the asymmetry of nascent strand template preference confirm that replication initiates non-randomly at multiple sites within 2.4 kb 5′ to the c-myc P1promoter, and at other sites over a region of 12 kb or more. The strongest template biases occur in the 2.4 kb region 5′ of the P1promoter, shown earlier to contain sequences which allow the autonomous semiconservative replication of c-myc plasmids. An asymmetric pyrimidine heptanucleotide consensus sequence has been identified which occurs 12 times in the c-myc origin zone, and whose polarity exactly correlates with the polarity of nascent strand synthesis.