Phosphorylated neurofilament subunit levels in the serum of cervical compressive myelopathy patients.
Phosphorylated neurofilament subunit levels in the serum of cervical compressive myelopathy patients.
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颈椎压迫性脊髓病患者血清中磷酸化神经丝亚基的水平。
DOI：
10.1016/j.jocn.2015.03.047
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ogata T
中科院分区：
文献类型：
--
作者：
Kato S;Chikuda H;Ohya J;Hayakawa K;Takeshita K;Tanaka S;Ogata T
我们研究了脊髓型颈椎病患者磷酸化形式的高分子量神经丝亚基 (pNF-H) 的血清水平。 pNF-H 越来越被认为是轴突损伤的生物标志物，然而，目前尚不清楚血清 pNF-H 在慢性脊髓受压时是否升高。我们检查了 26 名因颈椎压迫性脊髓病接受手术的患者。术前和术后 1 周采集外周血样本，使用酶联免疫吸附测定法评估血清 pNF-H 水平。还调查了近期脊髓病恶化的病史。 26 名脊髓病患者中，20 名患者术前血清 pNF-H 水平为阴性，6 名患者术前血清 pNF-H 水平中度升高。与 pNF-H 阴性患者相比，pNF-H 阳性患者近期脊髓病恶化的可能性更大（83% 与 25%；p = 0.02）。所有术前 pNF-H 阳性的患者术后仍保持阳性。两名手术后呈阳性的患者表现出与手术相关的神经系统恶化。总之，大多数脊髓型颈椎病患者血清 pNF-H 水平呈阴性。我们的结果表明，血清 pNF-H 水平升高与脊髓病的急性恶化有关，手术后 pNF-H 的阳性转化是围手术期神经损伤的标志。
We investigated the serum levels of the phosphorylated form of the high molecular weight neurofilament subunit (pNF-H) in patients with cervical compressive myelopathy. pNF-H is becoming increasingly recognized as a biomarker for axonal injury, however, it remains unclear whether serum pNF-H is elevated in chronic spinal cord compression. We examined 26 patients who underwent surgery for cervical compressive myelopathy. Peripheral blood samples were obtained both preoperatively and 1 week after surgery to evaluate the serum pNF-H levels using an enzyme-linked immunosorbent assay. A history of recent aggravation of myelopathy was also investigated. Of the 26 myelopathy patients, the preoperative serum pNF-H level was negative in 20 patients and moderately elevated in six. Patients who were positive for pNF-H were more likely to have had a recent aggravation of myelopathy compared with the pNF-H negative patients (83versus25%; p = 0.02). All patients who were positive for pNF-H before surgery remained positive after surgery. Two patients who became positive after surgery demonstrated a neurologic deterioration associated with the surgery. In conclusion, the serum pNF-H level was negative in the majority of patients with cervical compressive myelopathy. Our results suggest that an elevated serum level of pNF-H is associated with an acute worsening of myelopathy and that a positive conversion of pNF-H after surgery is a marker of perioperative neural damage.