Screening and identification of potential biomarkers in triple-negative breast cancer by integrated analysis
Screening and identification of potential biomarkers in triple-negative breast cancer by integrated analysis
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综合分析筛选和鉴定三阴性乳腺癌潜在生物标志物
DOI：
10.3892/or.2017.5911
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发表时间：
2017-10-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Bin
中科院分区：
文献类型：
--
作者：
Guo, Jilong;Gong, Guohua;Zhang, Bin
三阴性乳腺癌（TNBC）由于其独特的生物学特性、不良预后和侵袭性而引起人们的极大关注。TNBC患者更容易发生转移。我们筛选并鉴定了TNBC特异性基因作为潜在的生物标志物。在综合分析中使用了总共167个乳腺癌样品（45个TNBC和122个非TNBC）。应用基因表达谱芯片筛选差异表达基因。我们确定了65个核心DEG。通过GO和KEGG分析，揭示了TNBC中基因功能的富集，如基底细胞癌、前列腺癌、卵母细胞减数分裂和胆碱代谢等肿瘤通路。此外，PPI网络重建将有利于枢纽的筛选。还进行了TNBC特异性基因的RFS分析。RT-PCR用于验证TNBC中枢纽的表达模式。最后，共鉴定出9个基因，均为新的、特异性的、在TNBC中表达异常较高的基因。因此，这些基因将作为TNBC的潜在生物标志物，并有利于TNBC的进一步研究。
Triple-negative breast cancer (TNBC) has attracted great attention due to its unique biology, poor prognosis, and aggressiveness. TNBC patients are more likely to suffer from metastasis. We screened and identified the TNBC-specific genes as potential biomarkers. A total of 167 breast cancer samples (45 TNBC and 122 non-TNBC) were used in the integrated analysis. Gene expression microarrays were used to screen the differentially expressed genes. We identified 65 core DEGs. According to the GO and KEGG analysis, the gene function enrichment in TNBC was revealed, such as basal cell carcinoma, prostate cancer, oocyte meiosis and choline metabolism in cancer pathways. Moreover, the PPI network reconstruction would benefit the screening of hubs. A RFS analysis of TNBC-specific genes was also conducted. RT-PCR was used to validate the expression pattern of hubs in TNBC. Finally, nine genes were identified and all of them were novel, specific and higher dysregulation expressed genes in TNBC. Such that, these genes will serve as potential biomarkers in TNBC and benefit further research in TNBC.