Chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells in type I collagen-hydrogel for cartilage engineering
Chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells in type I collagen-hydrogel for cartilage engineering
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DOI：
10.1016/j.injury.2012.09.024
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发表时间：
2013-04-01
影响因子：
2.5
通讯作者：
Zeng, Yi
中科院分区：
文献类型：
--
作者：
Chen, Xuebin;Zhang, Fang;Zeng, Yi
导读：最近从人脐带（UC）沃顿氏胶中分离出一种强效间充质干细胞（MSC）群体。当前实验的目的是确定人uc来源的MSC （UC-MSC）在软骨愈合中的潜力。材料和方法：在标准软骨细胞调理培养基中诱导胶原水凝胶包埋细胞成软骨分化，并通过实时荧光定量PCR、组织化学和免疫组织化学分析检测。采用细胞凋亡检测试剂盒检测I型胶原水凝胶中MSCs的细胞活力和凋亡情况。结果：UC分离的细胞MSC生物标志物呈阳性，造血谱系和内皮细胞生物标志物呈阴性，并具有沿成骨谱系分化的能力。胶原水凝胶包埋的UC-MSCs可以发生软骨形成，其特征是将细胞包埋的水凝胶暴露于软骨形成因子后，胶原II、聚集蛋白、COMP（软骨寡聚基质蛋白）和sox9的表达显著增加。在软骨分化培养基中培养3周后，大部分细胞在整个水凝胶中保持活力。结论：胶原水凝胶可为UC-MSCs的软骨形成提供合适的三维环境。UC-MSCs包埋于生物相容性支架中，在软骨工程中具有很大的应用潜力。(C) 2012 Elsevier Ltd.版权所有。
Introduction: A potent mesenchymal stem cell (MSC) population was recently isolated from the Wharton's jelly of human umbilical cord (UC). The aim of the current experiments was to determine the potential of human UC-derived MSC (UC-MSC) in cartilage healing.Materials and methods: Chondrogenic differentiation of collagen hydrogel-embedded cells was induced in standard chondrocyte conditioning medium and further detected by real-time PCR, histochemistry and immunohistochemistry analyses. Cell viability and apoptosis of the MSCs in the collagen I hydrogels were monitored using apoptosis detection kit.Results: Cells isolated from UC were positive for MSC biomarkers and negative for haematopoietic lineage and endothelial biomarkers and possess the capacity to differentiate along osteogenic lineage. UC-MSCs embedded in collagen hydrogel can undergo chondrogenesis characterised by significantly increased expressions of collagen II, aggrecan, COMP (cartilage oligomeric matrix protein) and sox9 after exposed cells-embedded hydrogels to chondrogenic factors. The most of cells remained viable throughout the hydrogels after 3 weeks of cultivation in chondrogenic differentiation medium.Conclusions: Collagen hydrogel can provide an appropriate 3-D environment for the chondrogenesis of UC-MSCs. UC-MSCs embedded in biocompatible scaffold may have great potential for cartilage engineering. (C) 2012 Elsevier Ltd. All rights reserved.