Effect of lipid shell composition in DSPG-based microbubbles on blood flow imaging with ultrasonography
Effect of lipid shell composition in DSPG-based microbubbles on blood flow imaging with ultrasonography
复制标题
DOI：
10.1016/j.ijpharm.2020.119886
复制
发表时间：
2020-11-30
影响因子：
5.8
通讯作者：
Suzuki, Ryo
中科院分区：
文献类型：
--
作者：
Maruyama, Tamotsu;Sugii, Mutsumi;Suzuki, Ryo
诊断超声是非侵入性的，并提供实时成像。微泡（MB）是用于观察小血流（如肿瘤组织）的超声造影剂。然而，MB具有短的血流成像时间。本研究开发了基于脂质的微泡（LMBs），通过关注其外壳组成，具有更长的血流成像时间。脂质体研究报道，向脂质组合物中添加1，2-二硬脂酰-sn-甘油基-3-磷酸甘油（DSPG）增强脂质体膜稳定性。因此，我们将不同比例的DSPG引入LMB脂质壳中。结果表明，MBs的脂质壳组成影响体内稳定性。60%含DSPG的LMB（DSPG 60-LMB）与具有其他DSPG比率的LMB（Sonazoid（R）和SonoVue（R））相比具有持续的血流成像时间。与Sonazoid（R）相比，DSPG 60-LMB也显示出较少的肝脏摄取。因此，DSPG 60-LMB可以具有长的血流成像时间，并且可以是超声成像中的有效诊断剂。
Diagnostic ultrasound is non-invasive and provides real-time imaging. Microbubbles (MBs) are ultrasound contrast agents used to observe small blood flow, such as tumor tissue. However, MBs have short blood flow imaging time. This study developed lipid-based microbubbles (LMBs) with longer blood flow imaging time by focusing on their shell composition. Liposome research reported that addition 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) to the lipid composition enhances liposome membrane stability. Therefore, we introduced DSPG at different ratios into the LMBs lipid shell. Results showed that the lipid shell composition of MBs affects stability in vivo. 60% DSPG-containing LMBs (DSPG60-LMBs) have sustained blood flow imaging time compared with LMBs, which have other DSPG ratios, Sonazoid (R) and SonoVue (R). DSPG60-LMBs also showed less uptake into the liver compared with Sonazoid (R). Therefore, DSPG60-LMBs can have long blood flow imaging time and can be effective diagnostic agents in ultrasound imaging.