Improving seed size, seed weight and seedling emergence in Camelina sativa by overexpressing the Atsob3-6 gene variant
Improving seed size, seed weight and seedling emergence in Camelina sativa by overexpressing the Atsob3-6 gene variant
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DOI：
10.1007/s11248-020-00208-9
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发表时间：
2020-08
影响因子：
3
通讯作者：
Pushpa Sharma Koirala;M. Neff
中科院分区：
文献类型：
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作者：
Pushpa Sharma Koirala;M. Neff
在低降水量农业区，苗林建设是影响作物产量的关键因素。对于小种子作物来说尤其如此，比如亚麻荠（Camelina sativa）和油菜（Brassica napus），它们需要种植在浅地。深播是可取的，这样种子可以获得土壤水分，大种子可以通过提供幼苗伸长所需的能量来提高出苗率和立木率。AHL（AT-Hook Containing, nuclear localization）基因在幼苗生长发育中起着重要作用。AHL蛋白包含两个结构单元，dna结合AT-hook基序和plant and prokaryotecon保守（PPC）结构域，这是蛋白-蛋白相互作用所必需的。我们之前的研究表明，atahl29 /SOB3（Suppressorof phytochromeB-4 #3）调控拟南芥（Arabidopsis thaliana）幼苗的发育。激活标记的atsob3过表达（Atsob3-D）抑制拟南芥光敏色素B突变体的长下胚轴表型。相比之下，过度表达的atsob3 -6变体（Atsob3-6-OX），具有无功能的AT-hook，赋予长下胚轴表型。在这项研究中，我们证明了atsob3 - dandatsob3 -6- oxin在芸苔、拟南芥和亚麻荠中调节种子大小和下胚轴长度的作用。在拟南芥中，与野生型相比，atsob3减少种子重量，而atsob3 -6- ox增加种子重量和大小。同样，atsob3 -6- ox转基因亚麻荠幼苗比野生型高，并且产生更大更重的种子。与野生型相比，这些较大的atsob3 -6- ox亚麻荠种子在深土种植中也具有更好的出苗率。综上所述，atsob3 -6- ox增加油料作物亚麻荠种子大小、种子重量、幼苗下胚轴长度和林分建立。
Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such asCamelina(Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access soil moisture and bigger seeds could improve emergence and stand establishment by providing the energy necessary for seedling elongation.AHL(AT-Hook Containing, NuclearLocalized) genes play an important role in seedling growth and development. AHL proteins contain two structural units, the DNA-binding AT-hook motif and thePlant andProkaryoteConserved (PPC) domain, required for protein–protein interactions. Our previous studies demonstrate thatAtAHL29/SOB3(Suppressorof phytochromeB-4 #3) regulates seedling development inArabidopsis(Arabidopsis thaliana). Activation-tagged overexpression ofAtSOB3(Atsob3-D) represses the long-hypocotyl phenotype of anArabidopsisphytochrome B mutant. In contrast, overexpression of theAtsob3-6variant (Atsob3-6-OX), with a non-functional AT-hook, confers a long-hypocotyl phenotype. In this study, we demonstrate the role ofAtsob3-DandAtsob3-6-OXin modulating seed size and hypocotyl length in the brassicasArabidopsisandCamelina. InArabidopsis,Atsob3-Dreduces seed weight whereasAtsob3-6-OXincreases seed weight and size when compared to the wild type. Similarly,Atsob3-6-OXtransgenicCamelinaseedlings are taller than the wild type, and produce larger and heavier seeds. These largerAtsob3-6-OX Camelinaseeds also confer better emergence in deep-soil planting when compared to the wild type. Taken together,Atsob3-6-OXincreases seed size, seed weight, seedling hypocotyl length and stand establishment in the oilseed cropCamelina.