Enhanced recovery of low concentration protein and peptide solutions on ultra-low binding microplates
Enhanced recovery of low concentration protein and peptide solutions on ultra-low binding microplates
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DOI：
10.4155/fsoa-2018-0099
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发表时间：
2019-02-01
影响因子：
2.5
通讯作者：
Maurer, Brian R.
中科院分区：
文献类型：
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作者：
Weikart, Christopher M.;Breeland, Adam P.;Maurer, Brian R.
SiO2 Medical Products（SIO）开发了PureWARE超低结合（ULB）等离子体处理微孔板，具有提高蛋白质回收率和减少可提取物的综合优势。这项研究表明，与先前的研究相比，蛋白质回收率提高，但蛋白质浓度低十倍，即0.1 nM。此外，在环境储存条件下进行的26个月长期稳定性研究中，未观察到对等离子体处理微孔板蛋白回收率增强的显著影响。此外，三种不同的肽溶液的回收率，在1.5-12 nM的浓度范围内，也被证明是增强等离子体处理的微孔板相对于标准的聚丙烯microplate.Lay摘要：测试的治疗生物药物，称为生物分析测定，是针对了解药物是如何代谢的身体及其安全性。测定在聚合物微孔板中进行，该聚合物微孔板具有许多微型孔，如微型试管，其含有用于测试的药物溶液。生物药物分子因粘附在聚合物表面而臭名昭著。如果微孔板孔的液体中的生物量太低，则溶液中将没有剩余物可供检测。开发了一种新的涂层微孔板，以实现使用非常低浓度的生物分子进行测定，几乎没有粘附。
SiO2 Medical Products (SIO) developed PureWARE Ultra-Low Binding (ULB) plasma-treated microplates with the combined benefits of enhanced protein recovery and reduced extractables. This study demonstrates enhanced protein recoveries, but at ten-times lower protein concentration, or 0.1 nM, compared with a prior study. In addition, no significant effect on enhanced protein recovery of plasma-treated microplates was observed in a long-term stability study carried out for 26 months under ambient storage conditions. Furthermore, recovery of three different peptide solutions, in the concentration range of 1.5-12 nM, was also shown to be enhanced on plasma-treated microplates relative to standard polypropylene microplates.Lay abstract: Testing of therapeutic biologic drugs, called bioanalysis assays, is targeted at understanding how the drug is metabolized by the body and its safety. Assays are conducted in a polymer microplate that has many mini-wells, like miniature test tubes, that contain drug solution for testing. Biologic drug molecules are notorious for sticking to the polymer surface. If the amount of biologic in the liquid of a microplate well is too low, there will be nothing left in solution to test. A new coated microplate was developed to enable assays using very low concentrations of biologic molecules with little to no sticking.