ANTIMICROBIAL SUSCEPTIBILITY TESTING OF FRANCISELLA-TULARENSIS WITH A MODIFIED MUELLER-HINTON BROTH
ANTIMICROBIAL SUSCEPTIBILITY TESTING OF FRANCISELLA-TULARENSIS WITH A MODIFIED MUELLER-HINTON BROTH
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DOI：
10.1128/jcm.22.2.212-215.1985
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发表时间：
1985-01-01
影响因子：
9.4
通讯作者：
THORNSBERRY, C
中科院分区：
文献类型：
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作者：
BAKER, CN;HOLLIS, DG;THORNSBERRY, C
开发了一种改良的 Mueller-Hinton 肉汤，用于对土拉菌进行抗菌敏感性测试。接种后24小时内菌体充分生长，此时可读取最低抑菌浓度（MIC）。在含有 36 种抗菌剂的培养基中对 15 个选定的土拉弗朗西斯菌株和 5 个参考质量控制菌株进行了测试。与普通培养基相比，氨基糖苷类和四环素在该培养基中的 MIC 增加了 1 至 3 个稀释倍数，但其他抗菌药物并未一致受到该培养基的影响。尽管培养基导致 MIC 增加，但氨基糖苷类和四环素类药物在体外对土拉弗朗西斯菌仍然非常有效。其他体外有效的抗菌药物包括氯霉素、红霉素、头孢他啶、莫沙酰胺、头孢噻肟、头孢曲松和 Sch 29482（头孢菌素）。
A modified Mueller-Hinton broth was developed to perform antimicrobial susceptibility tests on F. tularensis. Adequate growth of the organism was obtained within 24 h of inoculation, and minimum inhibitory concentration (MIC) could be read at that time. The 15 selected strains of F. tularensis and 5 reference quality control strains were tested in this medium with 36 antimicrobial agents. The MIC of the aminoglycosides and tetracycline increased 1 to 3 dilutions in this medium compared with those in the usual medium, but the other antimicrobial agents were not consistently affected by the medium. Even though the medium caused an increase in MIC, the aminoglycosides and tetracyclines remained very active in vitro against F. tularensis. Other antimicrobial agents effective in vitro were chloramphenicol, erythromycin, ceftazidime, moxalactam, cefotaxime, ceftriaxone, and Sch 29482 (a cephalosporin).