IMPROVEMENT OF KASUGAMYCIN-PRODUCING STRAIN BY AGAR PIECE METHOD AND PROTOTROPH METHOD
IMPROVEMENT OF KASUGAMYCIN-PRODUCING STRAIN BY AGAR PIECE METHOD AND PROTOTROPH METHOD
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DOI：
10.1007/bf02884210
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发表时间：
1971-01-01
影响因子：
2.6
通讯作者：
OZAKI, A
中科院分区：
文献类型：
--
作者：
ICHIKAWA, T;DATE, M;OZAKI, A
发酵生产中抗生素产量的增加通常通过突变和选择生产菌株来实现。可以说，获得高产菌株的成功取决于筛选的继代培养物的数量。据报道，在链霉菌中，经诱变处理或未经诱变处理的突变频率为1/105 - 107（Mashima & Ikeda，1958）。但实际上，由于劳动力、空间和设备的限制，每周1，000个菌落是振荡培养所能测试的最大数量。当进行改良春雷霉素生产菌株的研究时，有必要提供一种允许定量比较大量菌落生产率的方法。测试了各种琼脂培养基方法。在一种传统方法中，切下在培养皿中的琼脂培养基上生长的每个菌落，立即置于生物测定板上并比较生产率。结果发现，这种方法不能应用于春雷霉素产生菌，因为如果在培养皿中的琼脂培养基上生长许多菌落，则一个菌落产生的春雷霉素迅速扩散到整个琼脂培养基上，结果不
The yield increase of an antibiotic in fermentation production has been achieved generally by mutation and selection of the producing strain. It can be said that success in obtaining the high-yield strain is dependent on the number of subcultures screened. It was reported that the frequency of mutation in streptomyces wi~ h or without mutagenic treatment is one per 105 to 107 (Mashima & Ikeda, 1958). But practically, 1,000 colonies per week is the largest number that can be tested by shaking culture, because of limitations of labor, space and equipment.When the study to improve the kasugamycin-producing strain was undertaken, it was necessary to provide a method permitting the quantitative comparison of a large number of colonies for productivity. Various agar media methods were tested. In one of the traditional methods, each colony growing on an agar medium in a Petri dish was cut out, immediately placed onto bioassay plate and the productivities were compared. It was found that this method could not be applied~ o the kasugamycin--producing strain, because if many colonies are grown on an agar medium in a Petri dish, kasugamycin produced by one colony rapidly diffuses all over the agar medium and the result does not