Serum 25-Hydroxyvitamin D and Adipose Tissue Vitamin D Receptor Gene Expression: Relationship With Obesity and Type 2 Diabetes
Serum 25-Hydroxyvitamin D and Adipose Tissue Vitamin D Receptor Gene Expression: Relationship With Obesity and Type 2 Diabetes
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DOI：
10.1210/jc.2014-3016
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发表时间：
2015-04-01
影响因子：
5.8
通讯作者：
Macias-Gonzalez, Manuel
中科院分区：
文献类型：
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作者：
Clemente-Postigo, Mercedes;Munoz-Garach, Araceli;Macias-Gonzalez, Manuel
背景：25-羟基维生素D[25(OH)D]与肥胖和2型糖尿病的关系尚不完全清楚。维生素D受体(VDR)在脂肪组织中的表达与肥胖有关，可能受1，25(OH)(2)D3[1，25(OH)(2)D3]的调节。目的：根据体重指数(BMI)和血糖状态分析AT患者血清25(OH)D和VDR基因的表达，并根据BMI分析1，25(OH)2D(3)对AT的影响。设计与患者：1)118例按BMI分为瘦、超重、肥胖、或病态肥胖[MO])及其血糖状态(正常血糖[NG]、糖尿病前期和糖尿病[P&D])；2)30例肥胖受试者(BMI和GT；30 kg/m(2))被分为NG和P&D两组。分析不同BMI供体AT外植体1，25(OH)(2)D-3体外刺激和前脂肪细胞分化过程中VDR基因的表达。地点：大学医院。主要观察指标：血清25(OH)D、甲状旁腺激素(PTH)和AT VDR基因表达。结果：P D组25(OH)D水平低于NG组，瘦肉组和MO组显著低于NG组(P<；0.05)。25(OH)D水平与胰岛素抵抗的稳态模型(HOMA-IR)(r=-0.200；P=.032)和血糖(r=-0.295；P=.001)呈负相关，但与体重指数无关。MO组VDR基因表达高于其他BMI组(P<；0.05)。1，25(OH)(2)D-3增加了肥胖患者AT中维生素D受体基因的表达(P<；0.05)，但不影响瘦体受试者。结论：与正常受试者相比，P&D患者的25(OH)D水平降低，与体重指数无关，并与糖代谢变量、suggestingthatvitaminDdeficiencyisassociatedmorewith糖代谢密切相关。此外，根据肥胖程度的不同，AT对1，25(OH)(2)D-3的反应也不同。
Context: The relationship between 25-hydroxyvitaminD[25(OH)D] andobesityandtype 2 diabetes is not completely understood. Vitamin D receptor (VDR) expression in adipose tissue (AT) is related to obesity and might be regulated by 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D3].Objective: To analyze serum 25(OH)D and VDR gene expression in AT according to body mass index (BMI) and glycemic status and the effect of 1,25(OH)2D(3) on AT according to BMI.Design and Patients: Two cohorts were studied: 1) 118 subjects classified according to their BMI (lean, overweight, obese, or morbidly obese [MO]) and their glycemic status (normoglycemic [NG] and prediabetic and diabetic [P& D]); and 2) 30 obese subjects (BMI > 30 kg/m(2)) classified as NG and P& D. VDR gene expression was analyzed during preadipocyte differentiation and in vitro stimulation with 1,25(OH)(2)D-3 of AT explants from donors with different BMI values.Setting: University Hospital.Main Outcome Measures: Serum 25(OH)D, parathyroid hormone (PTH), and AT VDR gene expression.Results: 25(OH)D levels were lower in P D than NG subjects, significantly so in the lean and MO groups (P < .05). 25(OH)D levels correlated negatively with homeostasis model of assessment for insulin resistance (HOMA-IR) (r = -0.200; P = .032) and glucose (r = -0.295; P = .001), but not with BMI. VDR gene expression was higher in MO than in the other BMI groups (P < .05). 1,25(OH)(2)D-3 increased VDR gene expression in AT from obese patients (P < .05) but not from lean subjects.Conclusions: 25(OH)D levels are diminished in P& D compared to NG subjects, independently of BMI, andare closelyrelatedtoglucosemetabolismvariables, suggestingthatvitaminDdeficiencyisassociatedmorewith carbohydrate metabolism than with obesity. Moreover, AT has a different response to 1,25(OH)(2)D-3 depending on the degree of obesity.