An electrochemical approach for detection of specific DNA-binding protein by gold nanoparticle-catalyzed silver enhancement.
An electrochemical approach for detection of specific DNA-binding protein by gold nanoparticle-catalyzed silver enhancement.
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DOI：
10.1016/j.ab.2007.12.006
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发表时间：
2008-04
影响因子：
2.9
通讯作者：
Qin Pan;Renyun Zhang;Yunfei Bai;N. He;Zuhong Lu
中科院分区：
文献类型：
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作者：
Qin Pan;Renyun Zhang;Yunfei Bai;N. He;Zuhong Lu
在生物系统中，转录因子与序列特异性DNA的相互作用在基因转录调控中起着重要作用。金（Au）纳米粒子作为电化学插层剂，在检测生物分子方面表现出较高的催化活性和相容性。在这篇文章中，我们报道了一种利用金纳米粒子催化银（Ag）增强电极和电解质溶液界面的电化学方法来检测序列特异性dna结合转录因子。将单分子发夹寡核苷酸自组装到Au电极表面，并在Au电极表面进行延伸，形成具有转录因子NF-κB（核因子κB）结合位点的双链寡核苷酸。采用阳极溶出伏安法（ASV）检测了金纳米颗粒催化银沉积对NF-κB结合的影响。结果表明，该方法对序列特异性dna结合蛋白的检测具有明显的特异性，检测限低至0.1 pM。结果表明，该方法在转录调控、操作位点识别和功能基因检测等方面具有一定的应用价值。
Interaction between transcription factor and sequence-specific DNA plays an important role in regulation of gene transcription in biological systems. As electrochemical intercalators, gold (Au) nanoparticles show high catalysis activity and compatibility for detection of biological molecules. In this article, we report an electrochemical approach for sequence-specific DNA-binding transcription factor detection by Au nanoparticle-catalyzed silver (Ag) enhancement at interface between electrodes and electrolyte solutions. Here unimolecular hairpin oligonucleotides were self-assembled onto Au electrode surface and their elongation on Au electrode surface was carried out to form double-stranded oligonucleotides with transcription factor NF-κB (nuclear factor–kappa B) binding sites. Au nanoparticle-catalyzed Ag deposition was detected by anodic stripping voltammetry (ASV) for NF-κB binding. It was found that this method for the detection of sequence-specific DNA-binding protein showed pronounced specificity and that the detection limit was as low as 0.1 pM. The findings indicated that our method can have applications in transcription regulation, operator site recognition, and functional gene inspection.