In vitro and in vivo activities of clarithromycin against the Mycobacterium avium complex.
In vitro and in vivo activities of clarithromycin against the Mycobacterium avium complex.
复制标题
克拉霉素对抗鸟分枝杆菌复合体的体外和体内活性。
DOI：
10.1016/0924-8579(94)90006-x
复制
发表时间：
1994
影响因子：
10.8
通讯作者：
S. Dekio
中科院分区：
文献类型：
--
作者：
H. Saito;H. Tomioka;Katsumasa Sato;S. Dekio
研究了克拉霉素（CAM）对多种分枝杆菌的体外抗菌活性。除forMycobacterium tuberculosis外，CAM对试验分枝杆菌的mic值与司帕沙星（SPFX）相当，但低于利福平（RMP）和氧氟沙星（OFLX）。0.25 μg/ml （1 MIC）浓度的CAM对m有杀菌作用。在7H9培养基和浓度分别为1或10 μg/ml（4和40 MIC）时，CAM对小鼠巨噬细胞中被吞噬的生物具有抗菌活性，而RMP则没有这种作用。将CAM给予s.c.或灌胃给感染m的小鼠。细胞内剂量为0.2 - 2mg /只，每天1次，每周6次，在降低肺部病变发生率和减少肺和脾脏细菌负荷方面表现出显著的治疗效果。
Clarithromycin (CAM) was assessed for in vitro antimicrobial activity against various mycobacterial species. Except forMycobacterium tuberculosis, CAM displayed MICs for test mycobacteria comparable to those of sparfloxacin (SPFX) but lower than those of rifampicin (RMP) and ofloxacin (OFLX). CAM at 0.25 μg/ml (1 MIC) exhibited bactericidal action againstM. intracellularegrowing in 7H9 medium and at 1 or 10 μg/ml (4 and 40 MIC, respectively) CAM displayed antimicrobial activity against the organism phagocytosed in murine macrophages, while RMP failed to manifest such an effect. When CAM was given s.c. or by gavage to mice infected i.v. withM. intracellulareat doses of 0.2 to 2 mg/mouse once daily, six times per week, it exhibited significant therapeutic efficacy in terms of decreased incidence of gross lung lesions and reduced bacterial loads in the lungs and spleen.