ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse
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DOI：
10.1016/s1074-7613(01)00224-2
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发表时间：
2001-11-01
期刊：
影响因子：
32.4
通讯作者：
Sperling, AI
中科院分区：
文献类型：
--
作者：
Allenspach, EJ;Cullinan, P;Sperling, AI
大粘蛋白CD 43被主动排除在T细胞/APC相互作用位点之外，集中在TCR接合位点远端的膜结构域中。CD 43的胞质区域是这种对足运动所必需和充分的。ERM细胞骨架衔接蛋白与CD 43共定位于该结构域。ERM显性阴性突变体阻断了CD 43和另一种已知的ERM结合蛋白Rho-GDI的远端积累。ERM功能的抑制降低了IL-2和IFN γ的产生，而不影响PKC θ聚焦或CD 69上调。这些结果表明，ERM蛋白组织一个复杂的远端的T细胞/APC相互作用的网站，并提供证据表明，充分的T细胞活化可能涉及从免疫突触的抑制蛋白的去除。
The large mucin CD43 is actively excluded from T cell/APC interaction sites, concentrating in a membrane domain distal to the site of TCR engagement. The cytoplasmic region of CD43 was necessary and sufficient for this antipodal movement. ERM cytoskeletal adaptor proteins colocalized with CD43 in this domain. An ERM dominant-negative mutant blocked the distal accumulation of CD43 and another known ERM binding protein, Rho-GDI. Inhibition of ERM function decreased the production of IL-2 and IFN gamma, without affecting PKC theta focusing or CD69 upregulation. These results indicate that ERM proteins organize a complex distal to the T cell/APC interaction site and provide evidence that full T cell activation may involve removal of inhibitory proteins from the immunological synapse.