GENETIC-CHARACTERIZATION OF HYPERRESISTANCE TO FORMALDEHYDE AND 4-NITROQUINOLINE-N-OXIDE IN THE YEAST SACCHAROMYCES-CEREVISIAE
GENETIC-CHARACTERIZATION OF HYPERRESISTANCE TO FORMALDEHYDE AND 4-NITROQUINOLINE-N-OXIDE IN THE YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00330602
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发表时间：
1988-02-01
期刊：
影响因子：
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通讯作者：
BRENDEL, M
中科院分区：
文献类型：
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作者：
MACK, M;GOMPELKLEIN, P;BRENDEL, M
用多拷贝质粒pAR172和pAR184转化的酵母菌株对4-硝基喹啉-n -氧化物（4-NQO）和甲醛（FA）的高抗性是由于这些质粒上存在SNQ和SFA两个基因。限制性内切分析显示SFA和SNQ的最大长度分别为2.7 kb和2.2 kb，其中包括转录控制元件。携带SFA的最小的2.7 kb亚克隆的存在使pAR184分离物对甲醛的抗性增加了5倍。pAR172分离物的小亚克隆对4-NQO的超抗性没有增加。与野生型相比，SFA基因的破坏导致对FA的敏感性增加了三倍。通过多拷贝载体导入单倍体酵母突变体rad2、rad3和snm1的基因SQO表达不能补充这些阻断切除修复的突变。
The hyperresistance to 4-nitroquinoline-N-oxide (4-NQO) and formaldehyde (FA) of yeast strains transformed with the multi-copy plasmids pAR172 and pAR184, respectively, is due to the two genes, SNQ and SFA, which are present on these plasmids. Restriction analysis revealed the maximal size of SFA as 2.7 kb and of SNQ as 2.2 kb, including transcription control elements. The presence of the smallest 2.7 kb subclone carrying SFA increased hyperresistance to formaldehyde fivefold over that of the original pAR184 isolate. No such increase in hyperresistance to 4-NQO was seen with the smaller subclones of the pAR172 isolate. Disruption of the SFA gene led to a threefold increase in sensitivity to FA as compared with the wild type. Expression of gene SQO introduced on a multi-copy vector into haploid yeast mutants rad2, rad3, and snm1 did not complement these mutations that block excision repair.