Streptococcal antagonism in oral biofilms:: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans
Streptococcal antagonism in oral biofilms:: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans
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DOI：
10.1128/jb.00276-08
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发表时间：
2008-07-01
影响因子：
3.2
通讯作者：
Herzberg, Mark C.
中科院分区：
文献类型：
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作者：
Kreth, Jens;Zhang, Yongshu;Herzberg, Mark C.
生物膜是多菌的，不同的细菌物种争夺有限的空间和营养。在健康的条件下，生物膜中的不同物种保持着生态平衡。这种平衡可能会受到环境因素和物种间相互作用的干扰。这些扰动会导致某些物种的优势生长，从而导致疾病。为了模拟临床上相关的种间拮抗，我们研究了三个特征良好且关系密切的口腔细菌，戈登链球菌、血链球菌和致龋性变形链球菌。血链球菌和戈登链球菌利用氧气的可用性和过氧化氢(H_2O_2)的不同产生来有效地与变形链球菌竞争。种间拮抗作用受葡萄糖的影响，过氧化氢产生减少。此外，好氧条件还刺激了变形链球菌的竞争能力系统和细菌素MUTACIN IV的表达，以及依赖于H_2O_2的异源DNA从血链球菌和戈登链球菌混合培养中的释放。这些数据为生态因素提供了新的见解，这些因素决定了先锋定植的口腔链球菌和口腔生物膜中变形链球菌的生存机制之间的竞争结果。
Biofilms are polymicrobial, with diverse bacterial species competing for limited space and nutrients. Under healthy conditions, the different species in biofilms maintain an ecological balance. This balance can be disturbed by environmental factors and interspecies interactions. These perturbations can enable dominant growth of certain species, leading to disease. To model clinically relevant interspecies antagonism, we studied three well-characterized and closely related oral species, Streptococcus gordonii, Streptococcus sanguinis, and cariogenic Streptococcus mutans. S. sanguinis and S. gordonii used oxygen availability and the differential production of hydrogen peroxide (H2O2) to compete effectively against S. mutans. Interspecies antagonism was influenced by glucose with reduced production of H2O2. Furthermore, aerobic conditions stimulated the competence system and the expression of the bacteriocin mutacin IV of S. mutans, as well as the H2O2-dependent release of heterologous DNA from mixed cultures of S. sanguinis and S. gordonii. These data provide new insights into ecological factors that determine the outcome of competition between pioneer colonizing oral streptococci and the survival mechanisms of S. mutans in the oral biofilm.