Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation.
Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation.
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盐古菌热激响应启动子调控机制的剖析：通用转录因子定向古菌基因调控的新范式
DOI：
10.1093/nar/gkn152
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发表时间：
2008-05
影响因子：
14.9
通讯作者：
Xiang H
中科院分区：
文献类型：
--
作者：
Lu Q;Han J;Zhou L;Coker JA;DasSarma P;DasSarma S;Xiang H
盐古菌中存在多种通用转录因子TBP和TFB，它们被认为是完成全局基因调控的关键因子。然而，GTF指导的转录调节在应激反应中的细节和作用仍不清楚。在这里，我们报告了一个全面的调查热诱导基因（热休克蛋白5）从盐杆菌的调节机制。我们通过突变分析证明，只要转录起始位点没有改变，hsp 5启动子（Phsp 5）核心元件（TATA box和BRE）的5′和3′序列对基础和热诱导基因表达没有显著影响。此外，BRE和TATA盒Phsp 5足以使非热响应启动子热诱导，在Haloferax volcano ii和盐杆菌属。NRC-1。DNA-蛋白质相互作用表明，两个热诱导的GTF，来自H. volcano ii和来自Halobacterium sp. NRC-1的TFBb可以以温度依赖性方式特异性结合Phsp 5。综上所述，Phsp 5的热响应性主要归因于核心启动子元件，这些元件在高温下被特异性热诱导的GTF有效识别，从而为GTF指导的基因调控提供了新的范例。
Multiple general transcription factors (GTFs), TBP and TFB, are present in many haloarchaea, and are deemed to accomplish global gene regulation. However, details and the role of GTF-directed transcriptional regulation in stress response are still not clear. Here, we report a comprehensive investigation of the regulatory mechanism of a heat-induced gene (hsp5) from Halobacterium salinarum. We demonstrated by mutation analysis that the sequences 5′ and 3′ to the core elements (TATA box and BRE) of the hsp5 promoter (Phsp5) did not significantly affect the basal and heat-induced gene expression, as long as the transcription initiation site was not altered. Moreover, the BRE and TATA box of Phsp5 were sufficient to render a nonheat-responsive promoter heat-inducible, in both Haloferax volcanii and Halobacterium sp. NRC-1. DNA–protein interactions revealed that two heat-inducible GTFs, TFB2 from H. volcanii and TFBb from Halobacterium sp. NRC-1, could specifically bind to Phsp5 likely in a temperature-dependent manner. Taken together, the heat-responsiveness of Phsp5 was mainly ascribed to the core promoter elements that were efficiently recognized by specific heat-induced GTFs at elevated temperature, thus providing a new paradigm for GTF-directed gene regulation in the domain of Archaea.
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影响因子：
2.8
作者：
Gregor, D;Pfeifer, F
通讯作者：
Pfeifer, F
DOI：
10.1073/pnas.86.14.5478
发表时间：
1989-07-01
影响因子：
11.1
作者：
LAM, WL;DOOLITTLE, WF
通讯作者：
DOOLITTLE, WF
影响因子：
5.6
作者：
Krüger, K;Hermann, T;Pfeifer, F
通讯作者：
Pfeifer, F
影响因子：
7
作者：
Falb, M;Pfeiffer, F;Oesterhelt, D
通讯作者：
Oesterhelt, D
影响因子：
7
作者：
Baliga, NS;Bonneau, R;Ng, WV
通讯作者：
Ng, WV