Deep sequencing analysis of transcriptomes in Aspergillus oryzae in response to salinity stress
Deep sequencing analysis of transcriptomes in Aspergillus oryzae in response to salinity stress
复制标题
米曲霉转录组响应盐度胁迫的深度测序分析。
DOI：
10.1007/s00253-017-8603-z
复制
发表时间：
2018-01-01
影响因子：
5
通讯作者：
Zeng, Bin
中科院分区：
文献类型：
--
作者：
He, Bin;Ma, Long;Zeng, Bin
表征各种刺激后的变化对于理解细胞对变化的条件的适应是至关重要的。米曲霉广泛应用于酱油工业生产，在盐度胁迫等复杂环境中往往会发生变化。然而，米曲霉对盐胁迫的保护生化机制却知之甚少。在这项研究中，我们成功地描述了米曲霉在盐胁迫下的发酵行为、转录图谱和代谢物的变化。结果表明，盐处理抑制了真菌的发育和分生孢子的形成。转录分析显示与精氨酸积累和油酸合成相关的基因表达上调。从转录组分析中获得的差异表达基因的可靠性和可用性进一步证实了qRT-PCR的结果。代谢组学分析表明，相应的精氨酸和油酸在细胞内的积累也随着盐胁迫的增加而增加。所有这些结果提供了稻瘟菌盐适应过程的整体转录组特征，并为通过基因工程提高耐盐性提供了多个靶基因。
Characterization of the changes after various stimuli is crucial to comprehend the adaptation of cells to the changing condition. Aspergillus oryzae is widely used for the industrial production of soy sauce, which always encounter changes within a complex environment, such as salinity stress. However, the protective biochemical mechanisms of A. oryzae against salinity stress are poorly understood. In this study, we successfully characterized the fermentative behavior, transcriptomic profiles, and metabolite changes of A. oryzae in response to salinity stress. The results showed that salt treatment of A. oryzae inhibited the fungal development and conidia formation. Transcriptomic analysis showed an upregulated expression of the genes related to arginine accumulation and oleic acid synthesis. The results of qRT-PCR were further confirmed by the reliability and availability of the differentially expressed genes obtained from the transcriptome analysis. Metabolomic analysis revealed that the corresponding intracellular accumulation of arginine and oleic acid were also increased in response to the salinity stress. All of the results provide a global transcriptome characterization of the salt adaptation process in A. oryzae, and offer multiple target genes for salt tolerance improvement via genetic engineering.