In vitro performance of lipid-PLGA hybrid nanoparticles as an antigen delivery system: lipid composition matters.
In vitro performance of lipid-PLGA hybrid nanoparticles as an antigen delivery system: lipid composition matters.
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脂质 - Plga杂交纳米颗粒作为抗原递送系统的体外性能：脂质组成很重要。
DOI：
10.1186/1556-276x-9-434
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发表时间：
2014
影响因子：
--
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Hu Y;Ehrich M;Fuhrman K;Zhang C
由于聚（乳酸-共-乙醇酸）（PLGA）纳米颗粒（NPs）和脂质体的许多有益特性，脂质-PLGA杂化纳米颗粒已被广泛研究作为癌症药物递送系统，生物成像剂载体，以及抗原递送载体。然而，脂质组成对脂质-PLGA混合纳米粒作为递送系统的性能的影响尚未得到很好的研究。在这项研究中，脂质组合物对混合纳米颗粒的稳定性和在不同条件下从纳米颗粒的体外抗原释放的影响进行了检查。详细研究了树突状细胞（DC）对具有各种表面电荷的杂合NP的摄取。结果表明，由具有更多正表面电荷的脂质壳包封的PLGA NPs可以提高杂化NPs的稳定性，使得包封在PLGA NPs中的抗原能够更好地受控释放，以及增强DC对NPs的摄取。
Due to the many beneficial properties combined from both poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and liposomes, lipid-PLGA hybrid NPs have been intensively studied as cancer drug delivery systems, bio-imaging agent carriers, as well as antigen delivery vehicles. However, the impact of lipid composition on the performance of lipid-PLGA hybrid NPs as a delivery system has not been well investigated. In this study, the influence of lipid composition on the stability of the hybrid NPs and in vitro antigen release from NPs under different conditions was examined. The uptake of hybrid NPs with various surface charges by dendritic cells (DCs) was carefully studied. The results showed that PLGA NPs enveloped by a lipid shell with more positive surface charges could improve the stability of the hybrid NPs, enable better controlled release of antigens encapsulated in PLGA NPs, as well as enhance uptake of NPs by DC.