Evidence for fumonisin inhibition of ceramide synthase in humans consuming maize-based foods and living in high exposure communities in Guatemala.
Evidence for fumonisin inhibition of ceramide synthase in humans consuming maize-based foods and living in high exposure communities in Guatemala.
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DOI：
10.1002/mnfr.201500499
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发表时间：
2015-11
影响因子：
5.2
通讯作者：
Gelineau-van Waes JB
中科院分区：
文献类型：
--
作者：
Riley RT;Torres O;Matute J;Gregory SG;Ashley-Koch AE;Showker JL;Mitchell T;Voss KA;Maddox JR;Gelineau-van Waes JB
伏马菌素（FB）存在于玉米中，是一种神经酰胺合成酶（CerS）抑制剂。我们测定了危地马拉高和低FB暴露社区中食用玉米的妇女血液中FB1 （UFB1）和鞘碱1-磷酸水平。使用UFB1来估计FB1摄入量。在尿液采集的同时用吸水纸采集血斑，测定鞘氨酸1-磷酸（Sa 1-P）、鞘氨酸1-磷酸（So 1-P）和鞘氨酸1-磷酸/So 1-P比值。在第一项研究中，每三个月（2011年3月至2012年2月）从生活在低（Chimaltenango和Escuintla）和高（Jutiapa） FB暴露社区的妇女（总共1240名新兵）收集血点和尿液。高FB1摄入群体血斑中UFB1、Sa 1-P/So 1-P比值和Sa 1-P/ml均显著高于低FB1摄入群体。2013年2月的一项后续研究证实了这一结果，该研究涉及299名生活在低（sacatepacimquez）和高（Santa Rosa和Chiquimula） FB暴露社区的妇女。高水平的FB1摄入与人体血液中Sa - 1-P和Sa - 1-P/So - 1-P比值的变化相关，与FB1抑制CerS的方式一致。
Fumonisin (FB) occurs in maize and is an inhibitor of ceramide synthase (CerS). We determined the urinary FB1 (UFB1) and sphingoid base 1-phosphate levels in blood from women consuming maize in high and low FB exposure communities in Guatemala. FB1 intake was estimated using the UFB1. Sphinganine 1-phosphate (Sa 1-P), sphingosine 1-phosphate (So 1-P), and the Sa 1-P/So 1-P ratio were determined in blood spots collected on absorbent paper at the same time as urine collection. In the first study, blood spots and urine were collected every three months (March 2011 to February 2012) from women living in low (Chimaltenango and Escuintla) and high (Jutiapa) FB exposure communities (1240 total recruits). The UFB1, Sa 1-P/So 1-P ratio, and Sa 1-P/ml in blood spots were significantly higher in the high FB1 intake community compared to the low FB1 intake communities. The results were confirmed in a follow-up study (February 2013) involving 299 women living in low (Sacatepéquez) and high (Santa Rosa and Chiquimula) FB exposure communities. High levels of FB1 intake are correlated with changes in Sa 1-P and the Sa 1-P/So 1-P ratio in human blood in a manner consistent with FB1 inhibition of CerS.