A novel angiomatoid epithelioid sarcoma cell line, Asra-EPS, forming tumors with large cysts containing hemorrhagic fluid in vivo.
A novel angiomatoid epithelioid sarcoma cell line, Asra-EPS, forming tumors with large cysts containing hemorrhagic fluid in vivo.
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DOI：
10.1186/1756-0500-6-305
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发表时间：
2013-08-01
影响因子：
1.8
通讯作者：
Yoshikawa H
中科院分区：
文献类型：
--
作者：
Imura Y;Naka N;Outani H;Yasui H;Takenaka S;Hamada K;Ozaki R;Kaya M;Yoshida K;Morii E;Myoui A;Yoshikawa H
虽然我们可以使用几个人上皮样肉瘤（ES）细胞系的基础和临床前研究，血管瘤样ES细胞系尚未报道。我们治疗了一例血管瘤样ES发展在右上肢的67岁男子。血管瘤样ES细胞系，Asra-EPS是新建立的，其特征在于其形态，生长速度和染色体分析。还在无胸腺裸鼠中分析Asra-EPS细胞的致瘤性。Asra-EPS细胞呈圆形、多角形或梭形，胞质丰富，在体外连续传代150代以上，传代时间超过15个月。这些细胞分泌癌抗原125（CA 125）、白细胞介素-6（IL-6）和血管内皮生长因子（VEGF）到培养基中。Asra-EPS细胞在植入裸鼠中时具有致瘤性，其中肿瘤在约50天时达到1000 mm 3的体积。在小鼠中形成的肿瘤的组织学特征与原始肿瘤的组织学特征基本相同，表现出嗜酸性上皮样和梭形细胞的多结节增殖，具有显著的出血区域和充满血液的囊性空间，与原始肿瘤中出血性囊肿形成的可能性显著对应。细胞角蛋白（AE 1/AE 3和CAM 5.2）、上皮膜抗原（EMA）、波形蛋白、CD 31、CD 34和CA 125免疫阳性，整合酶相互作用因子1（INI-1）和因子VIII相关抗原阴性。已建立的细胞系代表了一种生物学相关的新工具，以研究人类血管瘤样ES的分子病理学，并在体外和体内评估新疗法的疗效。
Whereas we can use several human epithelioid sarcoma (ES) cell lines for basic and preclinical research, an angiomatoid ES cell line has not been reported to date. We have treated a case of an angiomatoid ES developing in the right upper extremity of a 67-year-old man. An angiomatoid ES cell line, Asra-EPS was newly established and characterized for its morphology, growth rate and chromosomal analysis. Tumorigenicity of Asra-EPS cells was also analyzed in athymic nude mice. Asra-EPS cells were round, polygonal or spindle-shaped with an abundant cytoplasm and have been maintained continuously in vitro for over 150 passages during more than 15 months. These cells secreted cancer antigen 125 (CA 125), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) into the culture medium. Asra-EPS cells were tumorigenic when implanted in nude mice with tumors reaching a volume of 1000 mm3 at around 50 days. Histological features of tumors formed in mice were essentially the same as those of the original tumor, exhibiting a multinodular proliferation of eosinophilic epithelioid and spindle-shaped cells with prominent areas of hemorrhage and blood-filled cystic spaces strikingly corresponding to the potential of hemorrhagic cyst formation in the original tumor. They showed immunopositive staining for cytokeratins (AE1/AE3 and CAM5.2), epithelial membrane antigen (EMA), vimentin, CD31, CD34 and CA 125, but negative for integrase interactor 1 (INI-1) and factor VIII-related antigen. The established cell line represents a biologically relevant new tool to investigate the molecular pathology of human angiomatoid ES and to evaluate the efficacy of novel therapeutics both in vitro and in vivo.