Near-infrared fluorescence laparoscopy of the ureter with three preclinical dyes in a pig model
Near-infrared fluorescence laparoscopy of the ureter with three preclinical dyes in a pig model
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DOI：
10.1007/s00464-018-6596-z
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发表时间：
2019-03-01
影响因子：
3.1
通讯作者：
Stassen, Laurents P. S.
中科院分区：
文献类型：
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作者：
van den Bos, Jacqueline;Al-Taher, Mahdi;Stassen, Laurents P. S.
背景输尿管损伤据报道发生在1-7.6%的结直肠手术中。为了减少输尿管损伤的发生，识别输尿管是必要的。近红外荧光（NIRF）成像与静脉注射染料的使用可能是腹腔镜手术中输尿管可视化的附加价值。本研究的目的是评估三种临床前染料的性能; IRDye（（R））800 BK、IRDye（（R））800 NOS和IRDye（（R））800 CW，用于猪输尿管的近红外荧光腹腔镜检查。在每头猪中，使用浓度为1 mg/ml的6 mg静脉内剂量检测1种染料。使用腹腔镜成像系统在荧光模式和白色光模式下进行成像。为了进一步评估染料，离体成像实验进行，其中8个递减浓度的染料，稀释在PBS中，进行了评估，在一个透明的试管与NIRF模式在距离1，5和10厘米的腹腔镜。ResultsAll三种染料是有效的，允许识别输尿管与NIRF成像。IRDye（（R））800 BK、IRDye（（R））800 NOS和IRDye（（R））800 CW分别在35、45和10 min后输尿管变为荧光，最大靶背景比（TBR）分别为2.14、0.66和1.44。在离体成像实验中，所有三种染料在所有浓度和所有距离evaluated.ConclusionsIntravenous给药的临床前染料IRDye（（R））800 CW，IRDye（（R））800 BK和IRDye（（R））800 NOS促进成功识别活体猪模型中输尿管的解剖过程。使用IRDye（（R））800 BK时测得的TBR最高。在离体条件下，观察到信号的荧光强度与染料浓度以及与物体的距离之间的相关性。
BackgroundUreteric injury is reported to occur in 1-7.6% of colorectal surgeries. To reduce the incidence of ureteral injury, it is essential to identify the ureters. The use of near-infrared fluorescence (NIRF) imaging with intravenously administered dyes might be of added value for ureteral visualization during laparoscopy. The aim of this study is to assess the performance of three preclinical dyes; IRDye((R)) 800BK, IRDye((R)) 800NOS and IRDye((R)) 800CW, for near-infrared fluorescence laparoscopy of the ureter in pigs.MethodsIn three female Dutch landrace pigs, the new dyes were evaluated. In each pig, 1 dye was tested using a 6-mg intravenous dose in a concentration of 1mg/ml. Imaging was performed in fluorescence mode and white light mode with a laparoscopic imaging system. In order to further evaluate the dyes, an ex vivo imaging experiment was performed, in which 8 decreasing concentrations per dye, diluted in PBS, were evaluated in a transparent test tube with NIRF mode at a distance of 1, 5 and 10cm from the laparoscope.ResultsAll three dyes were effective in allowing the identification of the ureter with NIRF imaging. The ureter became fluorescent after 35, 45 and 10min, respectively, for IRDye((R)) 800BK, IRDye((R)) 800NOS and IRDye((R)) 800CW with a maximum target-to-background ratio (TBR) of 2.14, 0.66 and 1.44, respectively. In the ex vivo imaging experiment, all three dyes produced a strong fluorescence signal at all concentrations and all distances evaluated.ConclusionsIntravenous administration of the preclinical dyes IRDye((R)) 800CW, IRDye((R)) 800 BK and IRDye((R)) 800NOS facilitated successful identification of the anatomical course of the ureter in living pig models. The highest measured TBR occurred with the use of IRDye((R)) 800BK. Ex vivo, a correlation was observed between the fluorescence intensities of the signal with the concentration of the dye and with the distance to the object.