ELEMENTS OF A SINGLE MAP KINASE CASCADE IN SACCHAROMYCES-CEREVISIAE MEDIATE 2 DEVELOPMENTAL PROGRAMS IN THE SAME CELL-TYPE - MATING AND INVASIVE GROWTH
ELEMENTS OF A SINGLE MAP KINASE CASCADE IN SACCHAROMYCES-CEREVISIAE MEDIATE 2 DEVELOPMENTAL PROGRAMS IN THE SAME CELL-TYPE - MATING AND INVASIVE GROWTH
复制标题
DOI：
10.1101/gad.8.24.2974
复制
发表时间：
1994-12-15
影响因子：
10.5
通讯作者：
FINK, GR
中科院分区：
文献类型：
--
作者：
ROBERTS, RL;FINK, GR
缺乏氮的二倍体酿酒酵母菌株经历了从菌落生长形式到丝状假菌丝形式的发育转变。这种二型性需要一种极地萌发模式和单倍体交配信息素反应所必需的MAP激酶信号转导途径的元件。我们在这里报道，单倍体菌株表现出一种入侵生长行为，与假菌丝发育有许多相似之处，包括细丝形成和琼脂穿透。单倍体花丝的形成依赖于芽位选择从轴向两极模式的切换。在单倍体和二倍体中，花丝的形成都不同于琼脂的穿透。我们发现，二倍体假菌丝发育所需的MAP激酶级联的相同成分(STE20、STE11、STE7和STE12)也是单倍体中丝状体形成和琼脂穿透所必需的。因此，单倍体酵母细胞可以进入两种发育途径中的任何一种：交配或侵袭生长，这两种途径都取决于单个MAP激酶级联的元件。我们的结果为研究信号转导的动力学提供了一种新的发育模型，对高等真核生物具有重要意义。
Diploid Saccharomyces cerevisiae strains starved for nitrogen undergo a developmental transition from a colonial form of growth to a filamentous pseudohyphal form. This dimorphism requires a polar budding pattern and elements of the MAP kinase signal transduction pathway essential for mating pheromone response in haploids. We report here that haploid strains exhibit an invasive growth behavior with many similarities to pseudohyphal development, including filament formation and agar penetration. Haploid filament formation depends on a switch from an axial to a bipolar mode of bud site selection. Filament formation is distinct from agar penetration in both haploids and diploids. We find that the same components of the MAP kinase cascade necessary for diploid pseudohyphal development (STE20, STE11, STE7, and STE12) are also required for both filament formation and agar penetration in haploids. Thus, haploid yeast cells can enter either of two developmental pathways: mating or invasive growth, both of which depend on elements of a single MAP kinase cascade. Our results provide a novel developmental model to study the dynamics of signal transduction, with implications for higher eukaryotes.