MicroRNA and hepatitis C virus--challenges in investigation and translation: a review of the literature.
MicroRNA and hepatitis C virus--challenges in investigation and translation: a review of the literature.
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DOI：
10.1016/j.diagmicrobio.2014.05.024
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发表时间：
2014-09
影响因子：
2.9
通讯作者：
Holodniy, Mark
中科院分区：
文献类型：
--
作者：
Waldron, Paul Ravi;Holodniy, Mark
研究microRNA（miRNA）在丙型肝炎病毒（HCV）感染、疾病发病机制、宿主免疫和治疗反应中的作用有可能在诊断、预后和治疗方面产生创新。然而，在产生临床有用和可重复的结果方面仍然存在研究挑战。我们回顾了文献，主要侧重于用于构建我们目前对miRNA和HCV疾病的理解的方法和技术。第二个重点是了解潜在的临床研究应用，并澄清以前的研究结果。许多miRNA在跨多个组织区室的HCV感染的病毒和免疫发病机制中起关键作用。关于特定miRNA和HCV感染的相对测量、时间变化和生物学意义，已发表的研究中存在争议。为了协调不同的数据，额外的研究，最佳的样品处理，体外模型，微阵列差异表达的miRNA的技术，样品结果标准化的做法，和HCV基因型变异对表达的影响都是必要的。微阵列和miRNA分离技术的选择应基于在感兴趣的样品类型中产生可重复结果的能力。更直接地比较各种多重微阵列的有效性和可靠性以及围绕miRNA标准化和定量的改进共识是必要的，以便可以在研究之间比较数据。
Investigations into the role of microRNA (miRNA) in hepatitis C virus (HCV) infection, disease pathogenesis and host immune and treatment response have potential to produce innovations in diagnosis, prognosis and therapy. However, investigational challenges remain in generating clinically useful and reproducible results. We review the literature with a primary emphasis on methods and technologies used to construct our current understanding of miRNA and HCV disease. A second emphasis is to understand potential clinical research applications and provide clarification of previous study results. Many miRNA have key roles in viral and immunopathogenesis of HCV infection across multiple tissue compartments. Controversy exists among published studies regarding relative measurements, temporal changes and biological significance of specific miRNA and HCV infection. To reconcile diverging data, additional research into optimal sample processing, in vitro models, techniques for microarray differential expression of miRNAs, practices for sample result normalization, and effect of HCV genotype variation on expression are all necessary. Microarray and miRNA isolation techniques should be selected based on ability to generate reproducible results in the sample type of interest. More direct comparisons of efficacy and reliability of various multiplex microarrays and an improved consensus around miRNA normalization and quantitation are necessary so that data can be compared across studies.
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