Distribution and genetic diversity of the parasitic dinoflagellate Amoebophrya in coastal waters of China
Distribution and genetic diversity of the parasitic dinoflagellate Amoebophrya in coastal waters of China
复制标题
中国沿海寄生甲藻的分布及遗传多样性
DOI：
10.1016/j.hal.2019.101633
复制
发表时间：
2019-11-01
期刊：
影响因子：
6.6
通讯作者：
Li, Caiwen
中科院分区：
文献类型：
--
作者：
Chen, Tiantian;Xiao, Jie;Li, Caiwen
阿米巴原虫是一种专性体内寄生虫，感染沿海和海洋沃茨水域的多种海洋生物。由于其在海水中巨大的遗传多样性和对宿主甲藻生长的抑制作用，该寄生蜂受到越来越多的关注。由赤潮甲藻引起的赤潮对我国近海环境和海水养殖业造成了严重影响。在过去的几十年里，人们对赤潮的发生机制和危害进行了一系列的研究，但对赤潮消退的影响因素却鲜有研究。因此，本研究对我国沿海沿着的变形虫寄主范围、流行程度和多样性进行了系统调查，为进一步研究该寄生蜂的生态学作用奠定了基础。总共有10个甲藻类群被Amoebophrya spp.感染，患病率为0.03%~ 2.50%。环境基因组DNA测序结果显示，来自12个沿海海湾的Amoebophryidae序列的多样性和显著的区域异质性，而没有观察到显著的地理位置之间的相关性。系统发育分析的18 S rDNA序列来自个体Amoebophrya感染的细胞表明的寄生虫的主机分歧，并借给可信的多个物种的假设。研究结果进一步揭示了中国近海沃茨变形虫寄主范围广、分布广、多样性丰富的特点，以及该寄生蜂在海洋微生物食物网中的生态学意义。
Amoebophrya is an obligate endoparasite infecting wide ranges of marine organisms in coastal and oceanic waters. The parasitoid has received growing attention, due to its enormous genetic diversity in seawaters and suppressive effects on the growth of host dinoflagellates. Harmful algal blooms (HABs) caused by planktonic dinoflagellates have significantly impacted the coastal environment and mariculture in China. Series of studies have been conducted to reveal the occurrence mechanism and negative impacts of HABs in past decades, while the factors contributing to the recession of HABs have rarely been studied. Thus, the host range, prevalence and diversity of Amoebophrya along the coastline of China were systemically investigated to facilitate future studies on the ecological roles of the parasitoid. Overall, 10 dinoflagellate taxa were found to be infected by Amoebophrya spp., and the prevalence ranged from 0.03% to 2.50%. Sequencing of environmental genomic DNA revealed substantial diversity and significant regional heterogeneity of Amoebophryidae sequences derived from 12 coastal bays, while no significant correlation was observed among geographical locations. Phylogenetic analyses of 18S rDNA sequences derived from individual Amoebophrya-infected cells indicated the host divergence of the parasitoid and lend credence to the multiple species assumption. The results further revealed the broad host range, wide distribution and substantial diversity of Amoebophrya in the coastal waters of China, that should not be neglected in future studies on the succession of HABs, as well as the ecological significance of this parasitoid in marine microbial food webs.