Confocal reference free traction force microscopy.
Confocal reference free traction force microscopy.
复制标题
共聚焦参考游离牵引力显微镜。
DOI：
10.1038/ncomms12814
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发表时间：
2016-09-29
影响因子：
16.6
通讯作者：
Ferrari, Aldo
中科院分区：
文献类型：
--
作者：
Bergert, Martin;Lendenmann, Tobias;Zundel, Manuel;Ehret, Alexander E.;Panozzo, Daniele;Richner, Patrizia;Kim, David K.;Kress, Stephan J. P.;Norris, David J.;Sorkine-Hornung, Olga;Mazza, Edoardo;Poulikakos, Dimos;Ferrari, Aldo
细胞与其周围环境之间的机械连接对于组织发育、修复或病理过程中复杂生物过程的调节至关重要。牵引力显微镜（TFM）能够确定驱动力。尽管取得了进展，但需要克服低分辨率2D柱基方法中的侵入效应或高分辨率连续TFM方法中的细胞去除和衬底弛豫的破坏性中间步骤的重要限制。在这里，我们介绍了一种新的方法，允许一次（现场）采集连续的平面内和平面外的牵引场，具有高灵敏度。该方法基于将量子点电流体动力学纳米滴印到共焦单晶阵列中，在柔性基底上呈现可单独识别的点光源。我们证明了不受干扰的无参考的高分辨率连续力场的采集和定量，以及这种方法的同时能力，相关重叠牵引力与空间定位的蛋白质揭示使用免疫荧光方法。 牵引力显微镜是测量细胞与其环境之间的力的有效方法，但需要移除细胞以获得参考图像。在这里，作者使用纳米滴印刷的量子点到顺应性基板，以提供一个规则的基准点阵列，消除了对参考图像的需要。
The mechanical wiring between cells and their surroundings is fundamental to the regulation of complex biological processes during tissue development, repair or pathology. Traction force microscopy (TFM) enables determination of the actuating forces. Despite progress, important limitations with intrusion effects in low resolution 2D pillar-based methods or disruptive intermediate steps of cell removal and substrate relaxation in high-resolution continuum TFM methods need to be overcome. Here we introduce a novel method allowing a one-shot (live) acquisition of continuous in- and out-of-plane traction fields with high sensitivity. The method is based on electrohydrodynamic nanodrip-printing of quantum dots into confocal monocrystalline arrays, rendering individually identifiable point light sources on compliant substrates. We demonstrate the undisrupted reference-free acquisition and quantification of high-resolution continuous force fields, and the simultaneous capability of this method to correlatively overlap traction forces with spatial localization of proteins revealed using immunofluorescence methods. Traction force microscopy is an effective method of measuring forces between cells and their environment, but requires removing the cells to obtain a reference image. Here the authors use nanodrip printing of quantum dots into compliant substrates to provide a regular array of fiducial spots, removing the need for a reference image.
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影响因子：
10.8
作者：
Kress SJ;Antolinez FV;Richner P;Jayanti SV;Kim DK;Prins F;Riedinger A;Fischer MP;Meyer S;McPeak KM;Poulikakos D;Norris DJ
通讯作者：
Norris DJ
DOI：
10.1083/jcb.201108062
发表时间：
2012-04-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kim DH;Provenzano PP;Smith CL;Levchenko A
通讯作者：
Levchenko A
影响因子：
1.9
作者：
Horzum, Utku;Ozdil, Berrin;Pesen-Okvur, Devrim
通讯作者：
Pesen-Okvur, Devrim
影响因子：
2.7
作者：
Iwadate, Yoshiaki;Yumura, Shigehiko
通讯作者：
Yumura, Shigehiko
影响因子：
16.6
作者：
Galliker, P.;Schneider, J.;Poulikakos, D.
通讯作者：
Poulikakos, D.