Proteomic analysis of endoplasmic reticulum stress responses in rice seeds.
Proteomic analysis of endoplasmic reticulum stress responses in rice seeds.
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水稻种子内质网应激反应的蛋白质组学分析
DOI：
10.1038/srep14255
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发表时间：
2015-09-23
影响因子：
4.6
通讯作者：
Qu L
中科院分区：
文献类型：
--
作者：
Qian D;Tian L;Qu L
转基因水稻种子胚乳中贮藏蛋白分泌的缺陷往往导致内质网（内质网）应激，从而产生面粉状和皱缩的种子，但这种反应的机制尚不清楚。我们使用基于iTRAQ的蛋白质组学分析ER胁迫水稻种子由于胚乳特异性抑制OsSar 1，以确定响应ER胁迫的蛋白质水平的变化。与野生型对照相比，ER胁迫改变了水稻种子中405个蛋白质的表达，变化幅度大于2.0倍。在这些蛋白质中，有140种上调，265种下调。表达上调的蛋白质主要参与蛋白质的修饰、转运和降解，表达下调的蛋白质主要参与代谢和应激/防御反应。KOBAS分析表明，内质网中的蛋白质加工和降解相关的蛋白酶体是水稻胚乳响应内质网胁迫的主要上调途径。内质网应激反应中还涉及高尔基体蛋白的转运。结合生物信息学和分子生物学分析，我们的蛋白质组数据将有助于我们了解水稻种子对内质网胁迫的系统反应。
The defects in storage proteins secretion in the endosperm of transgenic rice seeds often leads to endoplasmic reticulum (ER) stress, which produces floury and shrunken seeds, but the mechanism of this response remains unclear. We used an iTRAQ-based proteomics analysis of ER-stressed rice seeds due to the endosperm-specific suppression of OsSar1 to identify changes in the protein levels in response to ER stress. ER stress changed the expression of 405 proteins in rice seed by >2.0- fold compared with the wild-type control. Of these proteins, 140 were upregulated and 265 were downregulated. The upregulated proteins were mainly involved in protein modification, transport and degradation and the downregulated proteins were mainly involved in metabolism and stress/defense responses. A KOBAS analysis revealed that protein-processing in the ER and degradation-related proteasome were the predominant upregulated pathways in the rice endosperm in response to ER stress. Trans-Golgi protein transport was also involved in the ER stress response. Combined with bioinformatic and molecular biology analyses, our proteomic data will facilitate our understanding of the systemic responses to ER stress in rice seeds.